Neuron-Specific Enolase as a Marker of in Vitro Neuronal Damage. Part I: Assessment of Neuron-Specific Enolase as a Quantitative and Specific Marker of Neuronal Damage
Hans, Pol; Bonhomme, Vincent; Collette, Julienet al.
1993 • In Journal of Neurosurgical Anesthesiology, 5 (2), p. 111-6
[en] Enolase in cerebrospinal fluid is a sensitive marker for many types of neurological injuries including head injury and ischemia. We assessed neuron-specific enolase (NSE) as a quantitative and specific biochemical marker of neuronal damage in an experimental model of kainate neurotoxicity. Rat hippocampal cultures were treated with various concentrations of kainate. NSE release into the culture medium was compared with neuronal death estimated either by direct cell counting or by lactate dehydrogenase (LDH) release, largely used to quantify neuronal injury. A dose-response relationship was observed between kainate concentration and the amount of NSE released (r = -0.69; p < 0.05) as well as a significant correlation between NSE release and neuronal death (r = 0.64; p < 0.05). Likewise, a significant correlation was found between LDH and NSE release (r = 0.85; p < 0.05). The specificity of NSE as an indicator of neuronal death was demonstrated using immunocyto-chemistry labeling and measurement of NSE release by pure astrocyte cultures. We concluded that NSE is a reliable, quantitative, and specific marker of neuronal injury.
Disciplines :
Anesthesia & intensive care
Author, co-author :
Hans, Pol ; Université de Liège - ULiège > Département des sciences cliniques > Anesthésie et réanimation
Bonhomme, Vincent ; Université de Liège - ULiège > Département des sciences cliniques > Département des sciences cliniques
Collette, Julien ; Centre Hospitalier Universitaire de Liège - CHU > Chimie médicale
Moonen, Gustave ; Université de Liège - ULiège > Département des sciences cliniques > Neurologie - Doyen de la Faculté de Médecine
Language :
English
Title :
Neuron-Specific Enolase as a Marker of in Vitro Neuronal Damage. Part I: Assessment of Neuron-Specific Enolase as a Quantitative and Specific Marker of Neuronal Damage
Publication date :
April 1993
Journal title :
Journal of Neurosurgical Anesthesiology
ISSN :
0898-4921
eISSN :
1537-1921
Publisher :
Lippincott Williams & Wilkins, United States - Maryland