Article (Scientific journals)
Gender differences in responses in Gammarus pulex exposed to BDE-47: a gel-free proteomic approach
Gismondi, Eric; Mazzucchelli, Gabriel; De Pauw, Edwin et al.
2015In Ecotoxicology and Environmental Safety, 122, p. 205-213
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Abstract :
[en] Very few ecotoxicological studies have considered differences in toxic effects on male and female organisms. Here, we investigated protein expression differences in caeca of Gammarus pulex males and females under control conditions (unexposed) and after 96 h exposure to BDE-47. Using gel-free proteomic analysis, we have identified 45 proteins, of which 25 were significantly differently expressed according to sex and/or BDE-47 exposure. These proteins were involved in several biological processes such as energy metabolism, chaperone proteins, or transcription/translation. In unexposed amphipods, 11 proteins were significantly over-expressed in females, and 6 proteins were over-expressed in males. Under BDE-47 stress, 7 proteins were differently impacted according to sex. For example, catalase was over-expressed in exposed females and under-expressed in exposed males, as compared to respective controls. Conversely, proteins involved in energy metabolism were up-regulated in males and down-regulated in females. Our proteomic study showed differences in responses of males and females to BDE-47 exposure, emphasizing that sex is a confounding factor in ecotoxicological assessment. However, due to the limited information existing in databases on Gammarids, it was difficult to define a BDE-47 mechanism of action. The gel-free proteomic seems to be a promising method to develop in future ecotoxicological studies and thus, to improve our understanding of the mechanism of action of xenobiotics.
Disciplines :
Environmental sciences & ecology
Author, co-author :
Gismondi, Eric ;  Université de Liège > Département de Biologie, Ecologie et Evolution > Ecologie animale et écotoxicologie
Mazzucchelli, Gabriel  ;  Université de Liège > Center for Analytical Research and Technology (CART)
De Pauw, Edwin  ;  Université de Liège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.)
Joaquim-Justo, Célia ;  Université de Liège > Département de Biologie, Ecologie et Evolution > Ecologie animale et écotoxicologie
Thomé, Jean-Pierre ;  Université de Liège > Département de Biologie, Ecologie et Evolution > Ecologie animale et écotoxicologie
Language :
English
Title :
Gender differences in responses in Gammarus pulex exposed to BDE-47: a gel-free proteomic approach
Publication date :
December 2015
Journal title :
Ecotoxicology and Environmental Safety
ISSN :
0147-6513
eISSN :
1090-2414
Publisher :
Academic Press, Orlando, United States - Florida
Volume :
122
Pages :
205-213
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 10 August 2015

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