Article (Scientific journals)
Characterization of a novel aphid prenyltransferase displaying dual geranyl/farnesyl diphosphate synthase activity
Vandermoten, Sophie; Charloteaux, Benoît; Santini, S. et al.
2008In FEBS Letters, 582 (16), p. 2471-2471
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Keywords :
Aphid; Isoprenyl diphosphate synthase; Geranyl diphosphate synthase; Farnesyl diphosphate synthase; Bifunctional enzyme; Homology Modelling
Abstract :
[en] We report on the cDNA cloning and characterization of a novel short-chain isoprenyl diphosphate synthase from the aphid Myzus persicae. Of the three IPPS cDNAs we cloned, two yielded prenyltransferase activity following expression in Escherichia coli; these cDNAs encode identical proteins except for the presence, in one of them, of an N-terminal mitochondrial targeting peptide. Although the aphid enzyme was predicted to be a farnesyl diphosphate synthase by BLASTP analysis, rMpIPPS, when isopentenyl diphosphate and dimethylallyl diphosphate are supplied as substrates, typically generated geranyl diphosphate (C10) as its main product, along with significant quantities of farnesyl diphosphate (C15). Analysis of an MpIPPS homology model pointed to substitutions that could confer GPP/FPP synthase activity to the aphid enzyme.
Disciplines :
Entomology & pest control
Author, co-author :
Vandermoten, Sophie ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech
Charloteaux, Benoît ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech
Santini, S.
Sen, Se.
Beliveau, C.
Vandenbol, Micheline ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech
Francis, Frédéric  ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech
Brasseur, Robert ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech
Cusson, M.
Haubruge, Eric  ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech
Language :
English
Title :
Characterization of a novel aphid prenyltransferase displaying dual geranyl/farnesyl diphosphate synthase activity
Publication date :
2008
Journal title :
FEBS Letters
ISSN :
0014-5793
eISSN :
1873-3468
Publisher :
Wiley-Blackwell, United States
Volume :
582
Issue :
16
Pages :
2471-2471
1928–1934
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 11 August 2009

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