Article (Scientific journals)
Darkness improves growth and delays necrosis in a nonchlorophyllous habituated sugarbeet callus: Biochemical changes
Kevers, Claire; Bisbis, B.; Le Dily, F. et al.
1995In In Vitro Cellular and Developmental Biology. Plant, 31 (2), p. 122-126
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Keywords :
Beta vulgaris
Abstract :
[en] The transfer of light-cultured green normal (N) and white habituated (HNO) sugarbeet callus to darkness reduced the growth of N callus and improved growth and delayed necrosis in the HNO callus. The decrease of dry matter of N callus under darkness was accompanied by a reduced content of carotenoids and by decreased CO2 fixation, which was compensated by an increased dependency on externally supplied sucrose. The levels of some organic nitrogen compounds such as glutamate, proline, and free polyamines were not affected by transfer to darkness of N or HNO callus. Darkness decreased ethylene emissions in both callus types. In the HNO callus, the sucrose growth dependency and the CO2 fixation were unaffected by darkness. Chlorophylls were absent both in light and darkness, whereas some carotenoids were accumulated in the HNO callus only in dark conditions. In another connection, a significant increase of peroxidase activity, which did not occur in the N callus, was induced by darkness in the HNO callus. A decreased content of thio-barbituric acid (TBA)-reactive substances was measured in the HNO callus transferred to darkness, whereas an increase was noticed in the N callus placed in the same conditions. These metabolic changes and the reduction of cellular damage in darkness revealed light-induced stress reactions leading to necrosis and to reduced growth of HNO callus. It appeared that darkness allowed the HNO callus to avoid the photooxidation stress. Therefore, the favorable effect of darkness on HNO growth might be explained by the suppression of photooxidative damage due to the absence of carotenoids. The higher peroxidase activity in the HNO callus maintained in darkness raised the problem of heme synthesis in this heterotrophic callus. © 1995 Society for In Vitro Biology.
Disciplines :
Phytobiology (plant sciences, forestry, mycology...)
Author, co-author :
Kevers, Claire ;  Université de Liège > Département des sciences de la vie > Département des sciences de la vie
Bisbis, B.;  Hormonologie Végétale, Institut de Botanique B 22, Université de Liège, Sart Tilman, Liège, B-4000, Belgium
Le Dily, F.;  Physiologie Végétale, IRBA, Univ. de Caen, Caen, F-14032, France
Billard, J. P.;  Physiologie Végétale, IRBA, Univ. de Caen, Caen, F-14032, France
Huault, C.;  Physiologie Végétale, IRBA, Univ. de Caen, Caen, F-14032, France
Gaspar, Th;  Hormonologie Végétale, Institut de Botanique B 22, Université de Liège, Sart Tilman, Liège, B-4000, Belgium
Language :
English
Title :
Darkness improves growth and delays necrosis in a nonchlorophyllous habituated sugarbeet callus: Biochemical changes
Publication date :
1995
Journal title :
In Vitro Cellular and Developmental Biology. Plant
ISSN :
1054-5476
eISSN :
1475-2689
Publisher :
Springer, Heidelberg, Germany
Volume :
31
Issue :
2
Pages :
122-126
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 15 July 2015

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