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Poster (Scientific congresses and symposiums)
PRESPHOTO project - Preservation of microalgae in the BCCM collections
Crahay, Charlotte; Deprez, Karolien; Vanormelingen, Pieter et al.
2015ECCO XXXIV European Culture Collections as tools in research and biotechnology
 

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Keywords :
cryopreservation; microalgae
Abstract :
[en] The implementation of reliable preservation technologies of the biological resources is crucial for the management of the Biological Ressources Centers. PRESPHOTO, a BRAIN.be project, aims to develop and optimize new and cost-effective preservation techniques of photosynthetic microalgae (diatoms and cyanobacteria) in the two BCCM collections, BCCM/DCG and BCCM/ULC. This is a critical factor for the future growth and valorisation of these collections. Firstly, the traditional two-step cryopreservation technique will be adapted to photosynthetic microalgae strains. In particular, we will examine the effects of the culture conditions, the type and concentration of cryoprotectants, and the preservation temperature on the survival of microalgae. The encapsulation/dehydration technique as alternative to the two-step cryopreservation method will be also evaluated. Moreover, an independent validation of the developed protocols will be performed by the Culture Collection of Algae and Protozoa (UK) (subcontractor). Secondly, we will perform genome resequencing of selected strains to investigate the genetic changes induced by different cryopreservation techniques. Finally, a high-quality genomic DNA bank will be established and validated.
Disciplines :
Microbiology
Author, co-author :
Crahay, Charlotte ;  Université de Liège > Centre d'ingénierie des protéines
Deprez, Karolien;  Ghent University > Biology Department > Laboratory of Protistology and Aquatic Ecology
Vanormelingen, Pieter;  Ghent University > Biology Department > Laboratory of Protistology and Aquatic Ecology
Vyverman, Wim;  Ghent University > Biology Department > Laboratory for Protistology and Aquatic Ecology
Day, John D;  Scottish Marine Institute > Culture Collection for Algae and Protozoa
Wilmotte, Annick  ;  Université de Liège > Département des sciences de la vie > Physiologie et génétique bactériennes
Language :
English
Title :
PRESPHOTO project - Preservation of microalgae in the BCCM collections
Publication date :
May 2015
Event name :
ECCO XXXIV European Culture Collections as tools in research and biotechnology
Event organizer :
Institut Pasteur
Event place :
Paris, France
Event date :
from May 27th to 29th
Audience :
International
Name of the research project :
PRESPHOTO
Funders :
BELSPO - SPP Politique scientifique - Service Public Fédéral de Programmation Politique scientifique
Available on ORBi :
since 09 July 2015

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