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Isolation and identification of anthropogenic particles in fish stomachs by Raman spectroscopy: a new method
Collard, France; Gilbert, Bernard; Eppe, Gauthier et al.
2015In Organohalogen Compounds
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Keywords :
Anthropogenic particles; planktivorous fishes; microplastics; Raman spectroscopy
Abstract :
[en] Microplastic particles (MP) contaminate oceans and affect marine organisms in several ways. Ingestion combined with food intake is generally reported. However, data interpretation is often circumvented by the difficulty to separate MP from bulk samples. Visual examination is often used as one or the only step to sort these particles. However, color, size and shape are insufficient and often unreliable criteria. Here we present an isolation method of MP specially adapted to a subsequent analysis by Raman spectroscopy. This method avoids fluorescence problems allowing the identification anthropogenic particles (AP) from stomach contents of fish by Raman spectroscopy. It was validated with commercial samples of microplastics and cotton along with stomach contents from three different Clupeiformes fishes: Clupea harengus, Sardina pilchardus and Engraulis encrasicolus. The optimized digestion and isolation protocol showed no visible impact on microplastics and cotton particles while the spectroscopic analysis allowed precise identification of microplastics and textile fibers. This approach allowed us to isolate 35 particles. These were analyzed by Raman spectroscopy: eleven were microplastics and thirteen were made of cellulose or lignin, or both (mostly fibers). Some particles were not identified but contained artificial colorants. This isolation protocol will help to assess the presence, quantity and composition of AP in planktivorous fish stomachs.
Research center :
MARE - Centre Interfacultaire de Recherches en Océanologie - ULiège
AFFISH-RC - Applied and Fundamental FISH Research Center - ULiège
Disciplines :
Aquatic sciences & oceanology
Author, co-author :
Collard, France  ;  Université de Liège > Département de Biologie, Ecologie et Evolution > Morphologie fonctionnelle et évolutive
Gilbert, Bernard ;  Université de Liège > Département de chimie (sciences) > Département de chimie (sciences)
Eppe, Gauthier  ;  Université de Liège > Département de chimie (sciences) > Chimie analytique inorganique
Parmentier, Eric  ;  Université de Liège > Département de Biologie, Ecologie et Evolution > Morphologie fonctionnelle et évolutive
Das, Krishna  ;  Université de Liège > Département de Biologie, Ecologie et Evolution > Océanologie
Language :
English
Title :
Isolation and identification of anthropogenic particles in fish stomachs by Raman spectroscopy: a new method
Publication date :
August 2015
Event name :
DIOXIN 2015 - 35th International Symposium on Halogenated Persistent Organic Pollutants
Event date :
August 23rd to 28th, 2015
Audience :
International
Journal title :
Organohalogen Compounds
ISSN :
1026-4892
Publisher :
Federal Environmental Agency, Vienna, Austria
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
Available on ORBi :
since 07 July 2015

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