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Poster (Scientific congresses and symposiums)
Use of 454 pyrosequencing protocol for the assessment of cyanobacterial diversity
Stelmach Pessi, Igor; De Carvalho, Pedro; Laughinghouse, Haywood Dail et al.
2015Empowering Biodiversity Research
 

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Keywords :
diversity; cyanobacteria; 454 pyrosequencing
Abstract :
[en] A pilot run was carried out on Antarctic microbial mat samples in order to test different protocols for 454 pyrosequencing and bioinformatic analyses. An artificial community was assembled using22 cyanobacterial strains in two different abundance distributions. Bioinformatic analyses were carried out on the artificial communities using variations of the mothur and UPARSE pipelines. Moreover, different DNA extraction methods, technical replicates and biases arising from direct barcoded primer amplification were assessed using five microbial mat and soil crust samples. A great variation in richness estimates was observed using different bioinformatic pipelines, with UPARSE generating the most consistent results. On the other hand, community structures observed using different DNA extraction protocols were statistically similar. The same was observed for the technical replicates. Moreover, the use of barcoded primers did not influence the observed community structure.
Research center :
CIP - Centre d'Ingénierie des Protéines - ULiège
Disciplines :
Microbiology
Author, co-author :
Stelmach Pessi, Igor ;  Université de Liège > Centre d'ingénierie des protéines
De Carvalho, Pedro
Laughinghouse, Haywood Dail
Wilmotte, Annick  ;  Université de Liège > Département des sciences de la vie > Physiologie et génétique bactériennes
Language :
English
Title :
Use of 454 pyrosequencing protocol for the assessment of cyanobacterial diversity
Alternative titles :
[fr] Utilisation du pyroséquencage 454 pour l'évaluation de la diversité des cyanobactéries
Publication date :
May 2015
Number of pages :
A0
Event name :
Empowering Biodiversity Research
Event organizer :
Belgian Biodiversity Platform
Event place :
Brussels, Belgium
Event date :
21 mai 2015
Name of the research project :
CCAMBIO
Funders :
BELSPO - SPP Politique scientifique - Service Public Fédéral de Programmation Politique scientifique
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
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since 06 July 2015

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