Article (Scientific journals)
Osterix/Sp7 regulates biomineralization of otoliths and bone in medaka (Oryzias latipes).
Renn, Jörg; Winkler, C
2014In Matrix Biology, 34, p. 193-204
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Keywords :
osterix; sp7; bone; osteoblast; medaka; function; knock-down; Otolith
Abstract :
[en] Osterix/Sp7 is a zinc finger transcription factor and critical regulator of osteoblast differentiation,maturation and activity. Osterix expression has also been described in non-skeletal tissues but functional analyses are lacking. In the present study, we show that in the teleost model medaka, osterix is present as two alternatively spliced transcripts, osx_tv1 and osx_tv2. Knock-down of osx_tv1 and/or osx_tv2 results in mineralization loss in early intramembranous bones while cartilage formation ismostly unaffected. Formation of the parasphenoid, the earliestmineralized bone in themedaka skeleton, is impaired and fails to recover at later stages. Ossification of later bones, such as the operculum and cleithrum, is delayed but recovers during further development. In the axial skeleton, formation of the neural arches and centra is strongly delayed. In vivo analyses using osterix:nlGFP and osteocalcin:GFP transgenic medaka and whole mount in situ hybridization suggest that bone defects observed after knock-down of osterix are caused by a delay of osteoblast maturation and activity. Furthermore, we analyzed expression profile and function of osterix during ear and otolith formation. We show that osterix is expressed in otic placodes at the otic vesicle stage and that its knock-down results in a loss of otoliths. Taken together, we show that osterix is required for bone formation in a teleost fish and that its important regulatory functions are conserved between teleosts and mammals. Furthermore, we provide the first functional evidence for a role of Osterix in a non-skeletal tissue, i.e. the otoliths.
Disciplines :
Genetics & genetic processes
Author, co-author :
Renn, Jörg ;  Université de Liège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Winkler, C;  NationalUniversity of Singapore > Department of Biological Sciences
Language :
English
Title :
Osterix/Sp7 regulates biomineralization of otoliths and bone in medaka (Oryzias latipes).
Publication date :
07 January 2014
Journal title :
Matrix Biology
ISSN :
0945-053X
eISSN :
1569-1802
Publisher :
Gustav Fischer Verlag, Stuttgart, Germany
Volume :
34
Pages :
193-204
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
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