Article (Scientific journals)
Differential role of snail1 and snail2 zinc fingers in E-cadherin repression and epithelial to mesenchymal transition
Villarejo; Cortés-Cabrera, Alvaro; Molina Ortiz, Patricia et al.
2014In Journal of Biological Chemistry, 289 (2), p. 930-941
Peer Reviewed verified by ORBi
 

Files


Full Text
J. Biol. Chem.-2014-Villarejo-930-41.pdf
Publisher postprint (3.99 MB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
DNA-binding Protein; Zinc Finger; Repressor Protein; EMT; E-cadherin
Abstract :
[en] Snail1 (Snail) and Snail2 (Slug) are transcription factors that share a similar DNA binding structure of four and five C2H2 zinc finger motifs (ZF), respectively. Both factors bind specifically to a subset of E-box motifs (E2-box: CAGGTG/CACCTG) in target promoters like the E-cadherin promoter and are key mediators of epithelial-to-mesenchymal transition (EMT). However, there are differences in the biological actions, in binding affinities to E-cadherin promoter, and in the target genes of Snail1 and Snail2, although the molecular bases are presently unknown. In particular, the role of each Snail1 and Snail2 ZF in the binding to E-boxes and in EMT induction has not been previously explored. We have approached this question by modeling Snail1 and Snail2 protein-DNA interactions and through mutational and functional assays of different ZFs. Results show that Snail1 efficient repression and binding to human and mouse E-cadherin promoter as well as EMT-inducing ability require intact ZF1 and ZF2, while for Snail2, either ZF3 or ZF4 is essential for those functions. Furthermore, the differential distribution of E2-boxes in mouse and human E-cadherin promoters also contributes to the differential Snail factor activity. These data indicate a non-equivalent role of Snail1 and Snail2 ZFs in gene repression, contributing to the elucidation of the molecular differences between these important EMT regulators.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Villarejo
Cortés-Cabrera, Alvaro
Molina Ortiz, Patricia ;  Université de Liège > GIGA-R : Labo de génétique fonctionnelle
Portillo, Francisco
Cano, Amparo
Language :
English
Title :
Differential role of snail1 and snail2 zinc fingers in E-cadherin repression and epithelial to mesenchymal transition
Publication date :
10 January 2014
Journal title :
Journal of Biological Chemistry
ISSN :
0021-9258
eISSN :
1083-351X
Publisher :
American Society for Biochemistry and Molecular Biology, Baltimore, United States - Maryland
Volume :
289
Issue :
2
Pages :
930-941
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 23 June 2015

Statistics


Number of views
57 (6 by ULiège)
Number of downloads
2 (2 by ULiège)

Scopus citations®
 
118
Scopus citations®
without self-citations
118
OpenCitations
 
90

Bibliography


Similar publications



Contact ORBi