Article (Scientific journals)
Characterization of regulatory elements in the medaka osterix promoter required for osteoblast expression.
Renn, Jörg; Buettner, A; Chua, EPS et al.
2014In Journal of Applied Ichthyology, 30 (4), p. 652–660
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Keywords :
osterix; sp7; bone; osteoblast; medaka; promoter
Abstract :
[en] The zinc finger transcription factor Osterix/Sp7 is an essential regulator of osteoblastogenesis. In mammals, osterix expression is regulated by Runx2, Msx2 and Dlx5 but recent findings suggest that also retinoic acid plays an important role for osteoblast differentiation and function. Yet, how these and other factors act on the osterix promoter is largely unknown. Expression, knock-down and promoter analyses have indicated that the function of Osterix in osteoblasts is conserved in teleosts and mammals. In the present study, we have used the teleost medaka to identify and characterize a region containing potential retinoic acid response elements in the osterix promoter. We analysed whether this region is important for activity in osteoblasts in vivo, using transgenic medaka lines with modified osterix promoter regions. Promoter activity in vivo and in vitro revealed a short nucleotide sequence in the promoter with crucial positive regulatory function. Mutations of this element lead to a complete inactivation of the osterix promoter in osteoblasts and made it insensitive to retinoic acid treatment. The comparison with the regulatory regions of osterix in other species suggests that the function of this element is highly conserved in vertebrates.
Disciplines :
Genetics & genetic processes
Author, co-author :
Renn, Jörg ;  Université de Liège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Buettner, A;  National University of Singapore > Department of Biological Sciences
Chua, EPS;  Nanyang Technological University, Singapore > Division of Molecular & Cell Biology
Tay, FS;  Nanyang Technological University, Singapore > Division of Molecular & Cell Biology
Featherstone, M;  Nanyang Technological University, Singapore > Division of Molecular & Cell Biology
Winkler, C;  National University of Singapore > Department of Biological Sciences
Language :
English
Title :
Characterization of regulatory elements in the medaka osterix promoter required for osteoblast expression.
Publication date :
25 March 2014
Journal title :
Journal of Applied Ichthyology
ISSN :
0175-8659
eISSN :
1439-0426
Publisher :
Wiley-Blackwell, Berlin, Germany
Volume :
30
Issue :
4
Pages :
652–660
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 23 June 2015

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