Article (Scientific journals)
Induction of Photosynthetic Carbon Fixation in Anoxia Relies on Hydrogenase Activity and Proton-Gradient Regulation-Like1-Mediated Cyclic Electron Flow in Chlamydomonas reinhardtii.
Godaux, Damien; Bailleul, Benjamin; Berne, Nicolas et al.
2015In Plant Physiology, 168 (2), p. 648-58
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Keywords :
chlamydomonas; cyclic electron flow; hydrogenase
Abstract :
[en] The model green microalga Chlamydomonas reinhardtii is frequently subject to periods of dark and anoxia in its natural environment. Here, by resorting to mutants defective in the maturation of the chloroplastic oxygen-sensitive hydrogenases or in Proton-Gradient Regulation-Like1 (PGRL1)-dependent cyclic electron flow around photosystem I (PSI-CEF), we demonstrate the sequential contribution of these alternative electron flows (AEFs) in the reactivation of photosynthetic carbon fixation during a shift from dark anoxia to light. At light onset, hydrogenase activity sustains a linear electron flow from photosystem II, which is followed by a transient PSI-CEF in the wild type. By promoting ATP synthesis without net generation of photosynthetic reductants, the two AEF are critical for restoration of the capacity for carbon dioxide fixation in the light. Our data also suggest that the decrease in hydrogen evolution with time of illumination might be due to competition for reduced ferredoxins between ferredoxin-NADP(+) oxidoreductase and hydrogenases, rather than due to the sensitivity of hydrogenase activity to oxygen. Finally, the absence of the two alternative pathways in a double mutant pgrl1 hydrogenase maturation factor G-2 is detrimental for photosynthesis and growth and cannot be compensated by any other AEF or anoxic metabolic responses. This highlights the role of hydrogenase activity and PSI-CEF in the ecological success of microalgae in low-oxygen environments.
Disciplines :
Biochemistry, biophysics & molecular biology
Phytobiology (plant sciences, forestry, mycology...)
Author, co-author :
Godaux, Damien ;  Université de Liège > Département des sciences de la vie > Génétique et physiologie des microalgues
Bailleul, Benjamin ;  Université de Liège > Département des sciences de la vie > Génétique et physiologie des microalgues
Berne, Nicolas ;  Université de Liège > Département des sciences de la vie > Génétique et physiologie des microalgues
Cardol, Pierre  ;  Université de Liège > Département des sciences de la vie > Génétique et physiologie des microalgues
Language :
English
Title :
Induction of Photosynthetic Carbon Fixation in Anoxia Relies on Hydrogenase Activity and Proton-Gradient Regulation-Like1-Mediated Cyclic Electron Flow in Chlamydomonas reinhardtii.
Publication date :
2015
Journal title :
Plant Physiology
ISSN :
0032-0889
eISSN :
1532-2548
Publisher :
American Society of Plant Biologists, United States - Maryland
Volume :
168
Issue :
2
Pages :
648-58
Peer reviewed :
Peer Reviewed verified by ORBi
Commentary :
(c) 2015 American Society of Plant Biologists. All Rights Reserved.
Available on ORBi :
since 17 June 2015

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