The involvement of hydrogen-producing and ATP-dependent NADPH-consuming pathways in setting the redox poise in the chloroplast of Chlamydomonas reinhardtii in anoxia.
Adenosine Triphosphate/metabolism; Anaerobiosis; Chlamydomonas reinhardtii/metabolism; Chloroplasts/metabolism; Hydrogen/metabolism; NADP/metabolism; Oxidation-Reduction; Oxygen/metabolism; Photosystem I Protein Complex/metabolism; Anaerobic Glycolysis; Chlamydomonas; Electron Transfer; Hydrogenase; Oxidation-Reduction (Redox); Photosynthesis
Abstract :
[en] Photosynthetic microalgae are exposed to changing environmental conditions. In particular, microbes found in ponds or soils often face hypoxia or even anoxia, and this severely impacts their physiology. Chlamydomonas reinhardtii is one among such photosynthetic microorganisms recognized for its unusual wealth of fermentative pathways and the extensive remodeling of its metabolism upon the switch to anaerobic conditions. As regards the photosynthetic electron transfer, this remodeling encompasses a strong limitation of the electron flow downstream of photosystem I. Here, we further characterize the origin of this limitation. We show that it stems from the strong reducing pressure that builds up upon the onset of anoxia, and this pressure can be relieved either by the light-induced synthesis of ATP, which promotes the consumption of reducing equivalents, or by the progressive activation of the hydrogenase pathway, which provides an electron transfer pathway alternative to the CO2 fixation cycle.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Clowez, Sophie
Godaux, Damien ; Université de Liège > Département des sciences de la vie > Génétique et physiologie des microalgues
Cardol, Pierre ; Université de Liège > Département des sciences de la vie > Génétique et physiologie des microalgues
Wollman, Francis-Andre
Rappaport, Fabrice
Language :
English
Title :
The involvement of hydrogen-producing and ATP-dependent NADPH-consuming pathways in setting the redox poise in the chloroplast of Chlamydomonas reinhardtii in anoxia.
Publication date :
2015
Journal title :
Journal of Biological Chemistry
ISSN :
0021-9258
eISSN :
1083-351X
Publisher :
American Society for Biochemistry and Molecular Biology, United States - Maryland
Volume :
290
Issue :
13
Pages :
8666-76
Peer reviewed :
Peer Reviewed verified by ORBi
Commentary :
(c) 2015 by The American Society for Biochemistry and Molecular Biology, Inc.
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