Reference : Influence Of A New Axial Impeller On K(L)A And Xylanase Production By Penicillium Canesc...
Scientific journals : Article
Life sciences : Microbiology
Influence Of A New Axial Impeller On K(L)A And Xylanase Production By Penicillium Canescens 10-10c
Bakri, Yasser [> > > >]
Jacques, Philippe [> > > >]
Shi, Kui mailto [> > > >]
Thonart, Philippe mailto [Université de Liège - ULg > Département des sciences de la vie > Biochimie et microbiologie industrielles - Gembloux Agro-Bio Tech >]
Applied Biochemistry and Biotechnology
Yes (verified by ORBi)
[en] Hydrolases ; O-Glycosidases ; Thallophyta ; Fungi Imperfecti ; Volumetric mass transfer coefficient ; Oxygen transfer
[en] The effects of a new axial impeller (HTPG4) on oxygen volumetric transfer coefficient, KLa, and xylanase production by Penicillium canescens 10-10c were studied and compared for dual-impeller systems, one with one DT4 impeller below and one HTPG4 above (DT4-HTPG4) and one with two DT4 (DT4-DT4) impellers, in a 5-L bioreactor. The volumetric coefficient of oxygen transfer was measured in culture medium using a gassing-out method at different gassing rates and agitation speeds. We observed that the DT4-HTPG4 combination provided better KLa performance than the DT4-DT4 combination. The two combinations were also tested for their influence on xylanase production by a filamentous microorganism; P. canescens 10-10c. These experiments demonstrated that the DT4-HTPG4 combination impeller enhanced enzyme production up to 23% compared with the DT4-DT4 combination at an aeration rate of 1 vvm and an agitation speed of 600 rpm. The main cause for this difference is thought to be a higher shear stress generated by the DT4-DT4 combination, which damages the mycelium of P. canescens and decreases xylanase production.

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