Article (Scientific journals)
Soluble factors regulated by epithelial-mesenchymal transition mediate tumour angiogenesis and myeloid cell recruitment.
Suarez-Carmona, Meggy; Bourcy, Morgane; LESAGE, J et al.
2015In Journal of Pathology, 236 (4), p. 491-504
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Keywords :
angiogenesis; cancer; epithelial-to-mesenchymal transition; myeloid cells
Abstract :
[en] Epithelial-to-mesenchymal transition (EMT) programs provide cancer cells with invasive and survival capacities that might favor metastatic dissemination. Whilst signaling cascades triggering EMT have been extensively studied, the impact of EMT on the crosstalk between tumor cells and the tumor microenvironment remains elusive. We aimed to identify EMT-regulated soluble factors that facilitate the recruitment of host cells in the tumor. Our findings indicate that EMT phenotypes relate to the induction of a panel of secreted mediators, namely IL-8, IL-6, sICAM-1, PAI-1 and GM-CSF, and implicate the EMT-transcription factor Snail as a regulator of this process. We further show that EMT-derived soluble factors are pro-angiogenic in vivo (in the mouse ear sponge assay), ex vivo (in the rat aortic ring assay) and in vitro (in a chemotaxis assay). Additionally, conditioned medium from EMT-positive cells stimulates the recruitment of myeloid cells. In a bank of 40 triple-negative breast cancers, tumors presenting features of EMT were significantly more angiogenic and infiltrated by a higher quantity of myeloid cells compared to tumors with little or no EMT. Taken together, our results show that EMT programs trigger the expression of soluble mediators in cancer cells that stimulate angiogenesis and recruit myeloid cells in vivo, which might in turn favor cancer spread.
Disciplines :
Anatomy (cytology, histology, embryology...) & physiology
Author, co-author :
Suarez-Carmona, Meggy ;  Université de Liège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement
Bourcy, Morgane ;  Université de Liège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement
LESAGE, J
Leroi, Natacha ;  Université de Liège > Département des sciences biomédicales et précliniques > Biologie cellulaire et moléculaire appliquée à l'homme
Syne, Laidya
Blacher, Silvia ;  Université de Liège > Département des sciences biomédicales et précliniques > Biologie cellulaire et moléculaire appliquée à l'homme
Hubert, Pascale  ;  Université de Liège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques
Erpicum, Charlotte ;  Université de Liège > R&D Direction : Chercheurs ULiège en mobilité
Foidart, Jean-Michel ;  Université de Liège > Département des sciences cliniques > Gynécologie - Obstétrique
Delvenne, Philippe ;  Université de Liège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques
Birembaut, Philippe
Noël, Agnès ;  Université de Liège > Département des sciences biomédicales et précliniques > Biologie cellulaire et moléculaire appliquée à l'homme
Polette, Myriam
Gilles, Christine ;  Université de Liège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement
More authors (4 more) Less
Language :
English
Title :
Soluble factors regulated by epithelial-mesenchymal transition mediate tumour angiogenesis and myeloid cell recruitment.
Publication date :
2015
Journal title :
Journal of Pathology
ISSN :
0022-3417
eISSN :
1096-9896
Publisher :
John Wiley & Sons, Inc, Chichester, United Kingdom
Volume :
236
Issue :
4
Pages :
491-504
Peer reviewed :
Peer Reviewed verified by ORBi
Commentary :
This article is protected by copyright. All rights reserved.
Available on ORBi :
since 28 May 2015

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