Poster (Scientific congresses and symposiums)
Isolation of insecticidal proteins within the pea seeds (Pisum sativum L.)
Cuartero Diaz, Gaëtan; Francis, Frédéric; Portetelle, Daniel et al.
2006International Conference on Proteomics : Bridging the gap between gene expression and biological function
 

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Keywords :
lectin; Pisum sativum; aphicide effect
Abstract :
[en] Consequently with the pressure exerted by chemical pesticides on environment, and the awakening of politics, the demand for bio-pesticides is increasing. Nevertheless, supply is not sufficient, and moreover those products are not competing enough. In this context, the aim of this research is to set up a biological insecticide, which is economic, with vegetal proteins resulting from alimentary industry, here the pea, Pisum sativum L.. A group of proteins, which is quite easy to highlight, is present in relatively important proportions (2%) in pea seeds, it’s the lectins class. Insecticidal effects of lectins from different organisms have already been proved. Indeed, by binding to membrane glycosyl groups of digestive tract cells, lectins can be very toxic for a lot of insects. Thus initially we focus our investigations on Pisum sativum lectins (PSL). First, PSL have been localised within the industrial process among different extraction juices. Then, a chromatography has been performed on the selected juice with FPLC technology. Although the matrix used for this chromatography, sephadex G75, is a banal bed for gel filtration, it is in this case a real combination between classical gel filtration and affinity chromatography. Indeed due the particular properties of lectins, they fixed carbonyl group of the bed and have to be eluted after the filtration part with a solution of glucose. Then the collected fractions corresponding to UV-peaks on the chromatogram were separated by electrophoresis 2D and identified by mass spectrometry (ESI MS/MS) coupled with data bank investigations. Secondly bioassays using artificial diets have been developed on Myzus persicae in the aim to study the aphicid effects of theses fractions with rm and LC50.These estimators show significant mortality rates but also change in the fecundity and in the development of nymph.
Disciplines :
Biochemistry, biophysics & molecular biology
Entomology & pest control
Biotechnology
Author, co-author :
Cuartero Diaz, Gaëtan;  Faculté Universitaire des Sciences Agronomiques de Gembloux - FUSAGx > Entomologie fonctionnelle et évolutive
Francis, Frédéric  ;  Université de Liège - ULiège > Sciences agronomiques > Entomologie fonctionnelle et évolutive
Portetelle, Daniel ;  Université de Liège - ULiège > Chimie et bio-industries > Biologie animale et microbienne
Colinet, Frédéric ;  Faculté Universitaire des Sciences Agronomiques de Gembloux - FUSAGx > Biologie animale et microbienne
Haubruge, Eric  ;  Université de Liège - ULiège > Sciences agronomiques > Entomologie fonctionnelle et évolutive
Language :
English
Title :
Isolation of insecticidal proteins within the pea seeds (Pisum sativum L.)
Publication date :
October 2006
Event name :
International Conference on Proteomics : Bridging the gap between gene expression and biological function
Event place :
Luxembourg, Luxembourg
Event date :
du 10 octobre 2006 au 14 octobre 2006
Audience :
International
Name of the research project :
PEAVALOR - First Europe
Available on ORBi :
since 10 August 2009

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