Poster (Scientific congresses and symposiums)
PRESPHOTO – a project to improve the preservation of cyanobacteria and diatom cultures
Deprez, Karolien; Vanormelingen, Pieter; Crahay, Charlotte et al.
2014Belgian Society for microbiology meeting November 18, 2014 - Cell Signaling in Host-Microbe Interactions
 

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Abstract :
[en] The availability of biological material of guaranteed identity and quality in Biological Resource Centers is considered fundamental for scientific research and R&D, but depends heavily on adequate preservation methods. We present a new BRAIN-BE project on improving the preservation of two groups of photosynthetic microorganisms, cyanobacteria and diatoms, in two collections of the Belgian Co-ordinated Collections of Micro-organisms (BCCM). First, we will improve the cultivation success of diatoms from different habitats by testing different culture media. For organisms that keep resisting cultivation, we will develop DNA extraction and amplification, as well as morphological investigation based on single cells. Secondly, we will design and validate improved cryopreservation protocols for both diatoms and cyanobacteria, since cryopreservation is now the preferred method for the long-term storage of microalgal cultures. For that, the two-step cryopreservation method will be tested using several cryoprotectants and strains in different growth stages. Moreover, this will be compared with the encapsulation/dehydration method. For cyanobacteria, viability tests will be performed with vital dyes whereas diatoms’ survival will be assessed by PAM fluorometry. In addition, genome resequencing will be applied to determine the impact of the cryopreservation protocol(s) on genomic stability. Finally, a genomic DNA bank will be constructed and validated. This is highly complementary to preservation as living strains, given that some users require only genomic DNA and it may not be feasible to preserve the global microalgal species diversity as living cultures.
Disciplines :
Microbiology
Author, co-author :
Deprez, Karolien;  Ghent University > Biology Department > Laboratory of Protistology and Aquatic Ecology
Vanormelingen, Pieter;  Ghent University > Biology Department > Laboratory of Protistology and Aquatic Ecology
Crahay, Charlotte ;  Université de Liège > Centre d'ingénierie des protéines
Wilmotte, Annick  ;  Université de Liège > Département des sciences de la vie > Physiologie et génétique bactériennes
Day, John G;  Scottish Marine Institute > Culture Collection for Algae and Protozoa
Vyverman, Wim;  Ghent University > Biology Department > Laboratory for Protistology and Aquatic Ecology
Language :
English
Title :
PRESPHOTO – a project to improve the preservation of cyanobacteria and diatom cultures
Publication date :
18 November 2014
Event name :
Belgian Society for microbiology meeting November 18, 2014 - Cell Signaling in Host-Microbe Interactions
Event date :
18th November 2014
Name of the research project :
PRESPHOTO
Funders :
BELSPO - SPP Politique scientifique - Service Public Fédéral de Programmation Politique scientifique
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since 21 May 2015

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