Poster (Scientific congresses and symposiums)
Epidemiological aspects and genotypic characterization of strains of Microsporum audouinii isolated in the context of a Belgian National survey on anthropophilic tinea
SACHELI, Rosalie; Dekkers, Charlotte; DARFOUF, Rajae et al.
2015Sixth FEBS Advanced Lecture Course, Human Fungal Pathogens :Molecular Mechanisms of Host-Pathogen Interactions and Virulence
 

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Keywords :
Dermatophytes; Diversilab
Abstract :
[en] Objectives The last two years, clinical cases of tinea capitis caused by Microsporum audouinii (M. audouinii), have increased in Belgium. To better understand the emergence of this species in the population, the Belgian National Reference Center (NRC) for dermatophytes launched a national survey in 2013. Epidemiological aspects and genotypic characterization of the strains were included. Methods The study was conducted from March 2013 up to February 2014. All Belgian laboratories were asked to send M. audouinii strains isolated from hair to the NRC with a form to fill in including epidemiological informations. The fungal strains were identified by microscopy or ITS sequencing in case of doubt. The genotypic analysis was performed by the DiversiLab® system (bioMérieux) for DNA fingerprinting and analysis. Epidemiological informations were analyzed with the help of a biostatistician. Results Among the collected isolates, 117 strains have been currently confirmed as M. audouinii. Analysis of the epidemiological characteristics of the infected population shows that the main age category concerns 5-9 year-old children (64%, p< 0,0001) with a sex-ratio M/F of 1.97. Data concerning the geographic origin of the family have been obtained in only 33,6% of the cases. It reveals that strains have been mainly isolated from patients with a Belgian nationality (43,6%) suggesting bias in the data collection. The geographic origin of the remaining group includes several African countries such as Congo (20,61%), Guinea (12,8%) and Burundi (5,12%). The genotypic analysis led to the distinction of 6 genotypic variants of M. audouinii. One of these variants was exclusively recovered from South Belgium (11 strains). The major group was composed of 96 strains, well distributed in different Belgium locations. Two other groups of three strains each were close to the major group but the analysis of the spectral superposition showed some differences between these groups. The two last groups were clearly different from the major group but species identification was confirmed by ITS sequencing. Conclusion The results of the genomic analysis by Diversilab, show that several groups of M. audouinii isolates co-exist in Belgium providing evidence of genetic heterogeneity. However, no clear correlation could be established between the appartenance to a group and epidemiological factors, such as the age or ethnical origin. ________________________________________
Disciplines :
Microbiology
Author, co-author :
SACHELI, Rosalie  ;  Centre Hospitalier Universitaire de Liège - CHU > Microbiologie clinique
Dekkers, Charlotte
DARFOUF, Rajae ;  Centre Hospitalier Universitaire de Liège - CHU > Microbiologie clinique
ADJETEY BAHUN, Akolé ;  Centre Hospitalier Universitaire de Liège - CHU > Microbiologie clinique
GRAIDE, Hélène ;  Centre Hospitalier Universitaire de Liège - CHU > Microbiologie clinique
MEEX, Cécile ;  Centre Hospitalier Universitaire de Liège - CHU > Microbiologie clinique
DESCY, Julie ;  Centre Hospitalier Universitaire de Liège - CHU > Microbiologie clinique
HUYNEN, Pascale ;  Centre Hospitalier Universitaire de Liège - CHU > Microbiologie clinique
MELIN, Pierrette  ;  Centre Hospitalier Universitaire de Liège - CHU > Microbiologie clinique
ARRESE ESTRADA, Jorge ;  Centre Hospitalier Universitaire de Liège - CHU > Dermatopathologie
HAYETTE, Marie-Pierre ;  Centre Hospitalier Universitaire de Liège - CHU > Microbiologie clinique
Language :
English
Title :
Epidemiological aspects and genotypic characterization of strains of Microsporum audouinii isolated in the context of a Belgian National survey on anthropophilic tinea
Publication date :
May 2015
Event name :
Sixth FEBS Advanced Lecture Course, Human Fungal Pathogens :Molecular Mechanisms of Host-Pathogen Interactions and Virulence
Event organizer :
Christophe d'Enfert, Institut Pasteur, Carol Munro and Anita Sil, UCSF (USA)
Event place :
Nice, France
Event date :
16-22 mai 2015
Audience :
International
Available on ORBi :
since 08 May 2015

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