Cochlear supporting cell transdifferentiation and integration into hair cell layers by inhibition of ephrin-B2 signalling

Defourny, Jean; Mateo Sanchez, Susana; Schoonaert, Lies; Robberecht, Wim; Davy, Alice; Nguyen, Laurent; Malgrange, Brigitte

doi:10.1038/ncomms8017

Article (Scientific journals)

Cochlear supporting cell transdifferentiation and integration into hair cell layers by inhibition of ephrin-B2 signalling

Defourny, Jean; Mateo Sanchez, Susana; Schoonaert, Lies et al.

2015 • In Nature Communications

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/181146

DOI
10.1038/ncomms8017

PubMed
25923646

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Keywords :

inner ear; ephrin

Abstract :

[en] In mammals, cochlear sensory hair cells that are responsible for hearing are postmitotic and are not replaced after loss. One of the most promising strategies to regenerate hair cells is to identify and inhibit the factors preventing the conversion of adjacent non-sensory supporting cells into hair cells. Here we demonstrate that mammalian hair cells can be directly generated from supporting cells by inhibition of ephrin-B2 signalling. Using either ephrin-B2 conditional knockout mice, shRNA-mediated gene silencing or soluble inhibitors, we found that downregulation of ephrin-B2 signalling at embryonic stages results in supporting cell translocation into hair cell layers and subsequent switch in cell identity from supporting cell to hair cell fate. As transdifferentiation is here a result of displacement across boundary, this original finding presents the interest that newly generated hair cells directly integrate either hair cell layer, then would be likely more rapidly able to fit into functional circuitry.

Research center :

Giga-Neurosciences - ULiège

Disciplines :

Biochemistry, biophysics & molecular biology
Anatomy (cytology, histology, embryology...) & physiology
Otolaryngology

Author, co-author :

Defourny, Jean ; Institut Pasteur (Paris)

Mateo Sanchez, Susana ; Université de Liège > Département des sciences cliniques > Neurologie

Schoonaert, Lies; Katholieke Universiteit Leuven - KUL

Robberecht, Wim; Katholieke Universiteit Leuven - KUL

Davy, Alice; Université Paul Sabatier - Toulouse 3 - UPS

Nguyen, Laurent ; Université de Liège > GIGA - Neurosciences

Malgrange, Brigitte ; Université de Liège > GIGA - Neurosciences

Language :

English

Title :

Cochlear supporting cell transdifferentiation and integration into hair cell layers by inhibition of ephrin-B2 signalling

Publication date :

29 April 2015

Journal title :

Nature Communications

eISSN :

2041-1723

Publisher :

Nature Pub.lishing Group, London, United Kingdom

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

http://reflexions.ulg.ac.be/CellulesOreille
http://reflexions.ulg.ac.be/en/EarCells

Available on ORBi :

since 04 May 2015

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