Article (Scientific journals)
Gene Expression Study in Positron Emission Tomography–Positive Abdominal Aortic Aneurysms Identifies CCL18 as a Potential Biomarker for Rupture Risk
Courtois, Audrey; Nusgens, Betty; HUSTINX, Roland et al.
2015In Molecular Medicine
Peer reviewed
 

Files


Full Text
14_065_courtois.pdf
Publisher postprint (4.82 MB)
Download

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
abdominal aortic aneurysm; positron emission tomography; microarray
Abstract :
[en] Rupture of abdominal aortic aneurysm (AAA) is a cause of significant mortality and morbidity in ageing populations. Uptake of 18-fluorodeoxyglucose (FDG) detected by positron emission tomography (PET) is observed in the wall of 12% of AAA (A+), most of them being symptomatic. We previously showed that the metabolically active areas displayed adventitial inflammation, medial degeneration and molecular alterations prefacing wall rupture. The aim of this study was to identify new factors predictive of rupture.Transcriptomic analyses were performed in the media and adventitia layers from three types of samples: AAA without (A0) and with FDG uptake (A+), both at the positive spot (A+Pos) and at a paired distant negative site (A+Neg) of the same aneurysm. Follow-up studies included RT-PCR, immunohistochemical staining and ELISA. A large number of genes, including matrix metalloproteinases, collagens and cytokines as well as genes involved in osteochondral development, were differentially expressed in the A+Pos as compared to A+Neg. Moreover, a series of genes, notably CCL18, was differentially expressed both in the A+Neg and A+Pos as compared to the A0. A significant increase of CCL18 was also found at the protein level in the aortic wall and in peripheral blood of A+ patients as compared to A0.In conclusion, new factors, including CCL18, involved in the progression of AAA and, potentially, in their rupture were identified by a genome-wide analysis of PET-positive and negative human aortic tissue samples. Further work is needed to study their role in AAA destabilization and weakening.
Disciplines :
Surgery
Cardiovascular & respiratory systems
Author, co-author :
Courtois, Audrey ;  Université de Liège > Département des sciences cliniques > Chirurgie cardio-vasculaire et thoracique
Nusgens, Betty ;  Université de Liège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques
HUSTINX, Roland  ;  Centre Hospitalier Universitaire de Liège - CHU > Service médical de médecine nucléaire et imagerie onco
Namur, Gauthier
Gomez, Pierre
Kuivaniemi, Helena
Defraigne, Jean-Olivier ;  Université de Liège > Département des sciences cliniques > Chirurgie cardio-vasculaire et thoracique
Colige, Alain ;  Université de Liège > Département des sciences biomédicales et précliniques > Laboratoire des tissus conjonctifs
SAKALIHASAN, Natzi ;  Centre Hospitalier Universitaire de Liège - CHU > Chirurgie cardio-vasculaire
Language :
English
Title :
Gene Expression Study in Positron Emission Tomography–Positive Abdominal Aortic Aneurysms Identifies CCL18 as a Potential Biomarker for Rupture Risk
Publication date :
2015
Journal title :
Molecular Medicine
ISSN :
1076-1551
eISSN :
1528-3658
Publisher :
North Shore Long Island Jewish Research Institute, Manhasset, United States - New York
Peer reviewed :
Peer reviewed
Available on ORBi :
since 23 April 2015

Statistics


Number of views
67 (15 by ULiège)
Number of downloads
284 (9 by ULiège)

Scopus citations®
 
10
Scopus citations®
without self-citations
6
OpenCitations
 
9

Bibliography


Similar publications



Contact ORBi