Article (Scientific journals)
Genome-wide transcriptional analysis suggests hydrogenase- and nitrogenase-mediated hydrogen production in Clostridium butyricum CWBI 1009
Calusinska, Magdalena; Hamilton, Christopher; Monsieurs, Pieter et al.
2015In Biotechnology for Biofuels, 8 (27), p. 1-16
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Keywords :
Dark fermentation; Clostridium butyricum; [FeFe] hydrogenase; Nitrogenase; RNA-seq; 2D-DIGE
Abstract :
[en] Background: Molecular hydrogen, given its pollution-free combustion, has great potential to replace fossil fuels in future transportation and energy production. However, current industrial hydrogen production processes, such as steam reforming of methane, contribute significantly to the greenhouse effect. Therefore alternative methods, in particular the use of fermentative microorganisms, have attracted scientific interest in recent years. However the low overall yield obtained is a major challenge in biological H2 production. Thus, a thorough and detailed understanding of the relationships between genome content, gene expression patterns, pathway utilisation and metabolite synthesis is required to optimise the yield of biohydrogen production pathways. Results: In this study transcriptomic and proteomic analyses of the hydrogen-producing bacterium Clostridium butyricum CWBI 1009 were carried out to provide a biomolecular overview of the changes that occur when the metabolism shifts to H2 production. The growth, H2-production, and glucose-fermentation profiles were monitored in 20 L batch bioreactors under unregulated-pH and fixed-pH conditions (pH 7.3 and 5.2). Conspicuous differences were observed in the bioreactor performances and cellular metabolisms for all the tested metabolites, and they were pH dependent. During unregulated-pH glucose fermentation increased H2 production was associated with concurrent strong up-regulation of the nitrogenase coding genes. However, no such concurrent up-regulation of the [FeFe] hydrogenase genes was observed. During the fixed pH 5.2 fermentation, by contrast, the expression levels for the [FeFe] hydrogenase coding genes were higher than during the unregulated-pH fermentation, while the nitrogenase transcripts were less abundant. The overall results suggest, for the first time, that environmental factors may determine whether H2 production in C. butyricum CWBI 1009 is mediated by the hydrogenases and/or the nitrogenase. Conclusions: This work, contributing to the field of dark fermentative hydrogen production, provides a multidisciplinary approach for the investigation of the processes involved in the molecular H2 metabolism of clostridia. In addition, it lays the groundwork for further optimisation of biohydrogen production pathways based on genetic engineering techniques.
Research center :
CIP - Centre d'Ingénierie des Protéines - ULiège
Centre Wallon de Biologie Industrielle
Disciplines :
Energy
Microbiology
Biochemistry, biophysics & molecular biology
Biotechnology
Author, co-author :
Calusinska, Magdalena ;  Luxembourg Institute of Science and Technology > Environmental Research and Innovation Department
Hamilton, Christopher;  Université de Liège - ULiège > CWBI
Monsieurs, Pieter;  Belgian Nuclear Research Centre (SCK-CEN) > Microbiology Unit, Expertise Group for Molecular and Cellular Biology, Institute for Environment, Health and Safety
Mathy, Gregory;  Université de Liège - ULiège > Labo de Bioénergétique
Leys, Natalie;  Belgian Nuclear Research Centre (SCK-CEN) > Microbiology Unit, Expertise Group for Molecular and Cellular Biology, Institute for Environment, Health and Safety
Franck, Fabrice ;  Université de Liège - ULiège > Labo de Bioénergétique
Joris, Bernard ;  Université de Liège - ULiège > Département des sciences de la vie > Physiologie et génétique bactériennes
Thonart, Philippe ;  Université de Liège - ULiège > Chimie et bio-industries > Bio-industries
Hiligsmann, Serge ;  Université de Liège - ULiège > Chimie et bio-industries > Bio-industries
Wilmotte, Annick  ;  Université de Liège - ULiège > Département des sciences de la vie > Physiologie et génétique bactériennes
Language :
English
Title :
Genome-wide transcriptional analysis suggests hydrogenase- and nitrogenase-mediated hydrogen production in Clostridium butyricum CWBI 1009
Publication date :
2015
Journal title :
Biotechnology for Biofuels
eISSN :
1754-6834
Publisher :
BioMed Central, London, United Kingdom
Volume :
8
Issue :
27
Pages :
1-16
Peer reviewed :
Peer Reviewed verified by ORBi
Name of the research project :
Micro-H2 : bacterial and microalgal hydrogen production
Funders :
Action de Recherches concertées ARC 07/12 04- ULg- Communauté française
Available on ORBi :
since 17 April 2015

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