Article (Scientific journals)
Effects of transforming growth factor-beta 1 on human adrenocortical fasciculata-reticularis cell differentiated functions.
LEBRETHON, Marie-Christine; Jaillard, C.; Naville, D. et al.
1994In Journal of Clinical Endocrinology and Metabolism, 79 (4), p. 1033-9
Peer Reviewed verified by ORBi
 

Files


Full Text
Effects of Transforming Growth Factor-beta1 on Human Adrenocortical Fasciculata-Reticularis Cell Differentiated Functions.pdf
Publisher postprint (632.34 kB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
Adrenal Cortex/cytology/drug effects/physiology; Adrenocorticotropic Hormone/genetics/pharmacology; Cell Differentiation; Cells, Cultured; Dehydroepiandrosterone/analogs & derivatives/biosynthesis; Dehydroepiandrosterone Sulfate; Enzymes/genetics; Humans; Hydrocortisone/biosynthesis; RNA, Messenger/metabolism; Receptors, Angiotensin/metabolism; Receptors, Transforming Growth Factor beta/metabolism; Steroids/biosynthesis; Transforming Growth Factor beta/genetics/pharmacology
Abstract :
[en] Transforming growth factor-beta 1 (TGF beta 1) has been reported to have a strong inhibitory effect on the specific function of adrenal cells of several species. In the present study, we examined the effects of TGF beta 1 on cultured human fasciculata-reticularis cells. TGF beta 1 alone had no effect on ACTH receptor messenger ribonucleic acid (mRNA) levels and was unable to reduce the strong stimulatory effects of ACTH on its own receptor. However, TGF beta 1 enhanced angiotensin-II type 1 receptor mRNA and binding sites. Treatment with TGF beta 1 increased significantly the levels of 3 beta-hydroxysteroid dehydrogenase mRNA, reduced those of cytochrome P-450 17 alpha-hydroxylase mRNA, and had no effect on cholesterol side-chain cleavage cytochrome P-450 mRNA. Whatever the experimental condition, TGF beta 1 did not reduce basal or ACTH-stimulated cortisol production, but the production of dehydroepiandrosterone sulfate of TGF beta 1-treated cells was always decreased. The effects of TGF beta 1 on 3 beta-hydroxysteroid dehydrogenase mRNA and dehydroepiandrosterone sulfate were opposite the change observed at the time of adrenarche. As adrenal cells express TGF beta 1 mRNA, it is tempting to postulate that a local diminution of TGF beta 1 might be involved in adrenarche. Our findings also illustrate the specific species differences and, therefore, the caution to extrapolate to humans the results observed in other species.
Disciplines :
Endocrinology, metabolism & nutrition
Author, co-author :
LEBRETHON, Marie-Christine ;  Centre Hospitalier Universitaire de Liège - CHU > Pédiatrie
Jaillard, C.
Naville, D.
Begeot, M.
Saez, J. M.
Language :
English
Title :
Effects of transforming growth factor-beta 1 on human adrenocortical fasciculata-reticularis cell differentiated functions.
Publication date :
1994
Journal title :
Journal of Clinical Endocrinology and Metabolism
ISSN :
0021-972X
eISSN :
1945-7197
Publisher :
Oxford University Press, New York, United States - New York
Volume :
79
Issue :
4
Pages :
1033-9
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 16 April 2015

Statistics


Number of views
32 (1 by ULiège)
Number of downloads
0 (0 by ULiège)

Scopus citations®
 
60
Scopus citations®
without self-citations
54
OpenCitations
 
1

Bibliography


Similar publications



Contact ORBi