Article (Scientific journals)
The A5 gene of alcelaphine herpesvirus 1 encodes a constitutively active G-protei n-coupled receptor that is non-essential for the induction of malignant catarrhal fever in rabbits
Boudry, Christel; Markine-Goriaynoff, Nicolas; Delforge, Cédric et al.
2007In Journal of General Virology, 88 (Pt 12), p. 3224-3233
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Abstract :
[en] Many gammaherpesviruses encode G-protein-coupled receptors (GPCRs). Several in vivo studies have revealed that gammaherpesvirus GPCRs are important for viral replication and for virus-induced pathogenesis. The gammaherpesvirus alcelaphine herpesvirus 1 (AlHV-1) is carried asymptomatically by wildebeest, but causes malignant catarrhal fever (MCF) following cross-species transmission to a variety of susceptible species. The A5 ORF of the AlHV-1 genome encodes a putative GPCR. In the present study, we investigated whether A5 encodes a functional GPCR and addressed its role in viral replication and in the pathogenesis of MCF. In silico analysis supported the hypothesis that A5 could encode a functional GPCR as its expression product contained several hallmark features of GPCRs. Expression of A5 as tagged proteins in various cell lines revealed that A5 localizes in cell membranes, including the plasma membrane. Using [35S]GTPgammaS and reporter gene assays, we found that A5 is able to constitutively couple to alpha i-type G-proteins in transfected cells, and that this interaction is able to inhibit forskolin-triggered cAMP response element-binding protein (CREB) activation. Finally, using an AlHV-1 BAC clone, we produced a strain deleted for A5 and a revertant strain. Interestingly, the strain deleted for A5 replicated comparably to the wild-type parental strain and induced MCF in rabbits that was indistinguishable from that of the parental strain. The present study is the first to investigate the role of an individual gene of AlHV-1 in MCF pathogenesis.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Boudry, Christel ;  Université de Liège - ULiège > Immunologie et vaccinologie
Markine-Goriaynoff, Nicolas ;  Université de Liège - ULiège > Immunologie et vaccinologie
Delforge, Cédric 
Springael, J. Y.
de Leval, Laurence ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques
Drion, Pierre ;  Université de Liège - ULiège > Centre animalier universitaire - Animalerie centrale
Russel, G.
Haig, D. M.
Vanderplasschen, Alain 
Dewals, Benjamin G  ;  Université de Liège - ULiège > Immunologie et vaccinologie
Language :
English
Title :
The A5 gene of alcelaphine herpesvirus 1 encodes a constitutively active G-protei n-coupled receptor that is non-essential for the induction of malignant catarrhal fever in rabbits
Publication date :
2007
Journal title :
Journal of General Virology
ISSN :
0022-1317
eISSN :
1465-2099
Publisher :
Soc General Microbiology
Volume :
88
Issue :
Pt 12
Pages :
3224-3233
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 07 August 2009

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