Article (Scientific journals)
Microfluidic Enzymatic Biosensing Systems: a Review
Mross, Stefan; Pierrat, Sebastien; Zimmermann, Tom et al.
2015In Biosensors and Bioelectronics, 70, p. 376-391
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Abstract :
[en] Microfluidic biosensing systems with enzyme-based detection have been extensively studied in the last years owing to features such as high specificity, a broad range of analytes and a high degree of automation. This review gives an overview of the most important factors associated with these systems. In the first part, frequently used immobilization protocols such as physisorption and covalent bonding and detection techniques such as amperometry and fluorescence measurements are discussed with respect to effort, lifetime and measurement range. The Michaelis–Menten model describing the kinetics of enzymatic reactions, the role of redox mediators and the limitations of the linear measurement range of enzymatic sensors are introduced. Several possibilities of extending the linear measurement range in microfluidic systems such as diffusion-limiting membranes and the flow injection setup are presented. Regarding the integration of enzymes into microfluidic systems during the fabrication process, the constraints imposed by the biomolecules due to the limited usage of high temperatures and solvents are addressed. In the second part, the most common forms of enzyme integration into microfluidic systems, i.e. in channels and on electrodes, on microparticles, on paper and thread and as injected enzyme solutions, are reviewed, focusing on fabrication, applications and performance.
Disciplines :
Electrical & electronics engineering
Author, co-author :
Mross, Stefan
Pierrat, Sebastien
Zimmermann, Tom
Kraft, Michael ;  Université de Liège - ULiège > Dép. d'électric., électron. et informat. (Inst.Montefiore) > Systèmes microélectroniques intégrés
Language :
English
Title :
Microfluidic Enzymatic Biosensing Systems: a Review
Publication date :
2015
Journal title :
Biosensors and Bioelectronics
ISSN :
0956-5663
eISSN :
1873-4235
Publisher :
Elsevier, Oxford, United Kingdom
Volume :
70
Pages :
376-391
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 02 April 2015

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