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Molecular diversity of microorganisms in Antarctic lacustrine microbial mats
Wilmotte, Annick; Stelmach Pessi, Igor; Sweetlove, Maxime et al.
20152015 Aquatic Sciences Meeting 'Aquatic Sciences: global and regional perspectives - North meets South'
 

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Keywords :
Biogeography; Antarctica; benthic mats
Abstract :
[en] The BeSPO project CCAMBIO aims to study the biogeographical distribution of microorganisms in lacustrine microbial mats using a combination of techniques including microscopical observations (light and electronic), strain isolation, and molecular diversity assessment using Next Generation Sequencing. The samples were collected in different Antarctic and sub-Antarctic biogeographical regions. A multivariate analysis of diatoms showed that these regions hosted different diatom flora. Endemic diatom taxa were also observed, and a multigene molecular phylogeny of Pinnularia borealis showed a high genetic diversity. A new Scenedesmacean species was described from Antarctica, Chodatodesmus australis. A comparison of the bacterial diversity retrieved by cultivation or NGS showed a complementarity of both approaches and differences when different variable regions of the 16S rRNA gene were used. Novel and unclassified sequences, also observed by other authors, were obtained. Pilot studies were conducted for the microeukaryotes and cyanobacteria to select NGS protocols and bioinformatic pipelines. The purpose is to deposit the diversity data in the “Microbial Antarctic Resource System (MARS)” presently developed into the webportal ‘biodiversity.aq’.
Research center :
CIP - Centre d'Ingénierie des Protéines - ULiège
Disciplines :
Environmental sciences & ecology
Microbiology
Author, co-author :
Wilmotte, Annick  ;  Université de Liège - ULiège > Département des sciences de la vie > Physiologie et génétique bactériennes
Stelmach Pessi, Igor ;  Université de Liège - ULiège > Centre d'ingénierie des protéines
Sweetlove, Maxime
Obbels, Dagmar
Vanormelingen, Pieter
Tytgat, Bjorn
Willems, Anne
Verleyen, Elie
Vyverman, Wim
Van De Vijver, Bart
Lara, Yannick  ;  Université de Liège - ULiège > Centre d'ingénierie des protéines
Van De Putte, Anton
Language :
English
Title :
Molecular diversity of microorganisms in Antarctic lacustrine microbial mats
Publication date :
February 2015
Number of pages :
1
Event name :
2015 Aquatic Sciences Meeting 'Aquatic Sciences: global and regional perspectives - North meets South'
Event organizer :
Association for the Sciences of Limnology and Oceanography (ASLO)
Event place :
Grenada, Spain
Event date :
22-27 February 2015
Audience :
International
References of the abstract :
Program Book of 2015 Aquatic Sciences Meeting of ASLO
Name of the research project :
CCAMBIO
Funders :
BELSPO - SPP Politique scientifique - Service Public Fédéral de Programmation Politique scientifique
Available on ORBi :
since 02 April 2015

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