Article (Scientific journals)
HYDRODYNAMICS IN A DISPOSABLE RECTANGULAR PARALLELEPIPED STIRRED BIOREACTOR WITH ELLIPTIC PENDULUM MOTION PADDLE
Collignon, Marie-Laure; Droissart, Laurent; Delafosse, Angélique et al.
2015In Biochemical Engineering Journal, 93
Peer Reviewed verified by ORBi
 

Files


Full Text
Collignon_et_al, 2015.pdf
Author preprint (1.64 MB)
Download

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
Agitation; animal cell culture; bioprocess design; fluid mechanics; stereo-PIV; mean flow characterization
Abstract :
[en] Stainless steel bioreactors increasingly fall behind to their disposable counterparts in pharma research as they do not require cleaning or sterilization. This led company ATMI LifeSciences to develop the “Nucleo™”. Original in design, this disposable bioreactor comprises a rectangular parallelepiped plastic bag stirred by a paddle revolving in elliptic pendulum motion. Studies covering this bioreactor showed good homogeneity of culture medium as well as good productivity for animal cell cultures. To further explain these good performances, the flow inside the “Nucleo™” had to be resolved. This paper focuses on the mean flow description, computed from stereo-PIV measurements performed in 20 verticals covering the whole volume of a 50 dm3 Nucleo™ bioreactor. As the flow is already turbulent in the chosen agitation conditions, its dimensionless mean velocity field does not vary with the paddle rotational speed. Mean flow pattern exhibits an axial symmetry – same flow is observed in opposite quarters of the tank – and can be described as a three-dimensional helix coiled on itself to form a distorted horizontal torus which covers the whole tank volume. Mean velocity value is on average doubled in the cone swept by the paddle, and its two horizontal components are twice higher than its vertical ones. However, mean velocity remains significant everywhere and, in particular, no stagnant area is observed in tank corners. Our results thus confirm previous studies observations.
Disciplines :
Chemical engineering
Author, co-author :
Collignon, Marie-Laure ;  Université de Liège - ULiège > Département de chimie appliquée > Département de chimie appliquée
Droissart, Laurent;  Université de Liège - ULiège > Chimie Appliquée > Laboratoire de Génie Chimique
Delafosse, Angélique ;  Université de Liège - ULiège > Département de chimie appliquée > Génie de la réaction et des réacteurs chimiques
Calvo, Sébastien ;  Université de Liège - ULiège > Département de chimie appliquée > Génie chimique - Nanomatériaux et interfaces
Vanhamel, Steven;  ATMI LifeSciences
Rodriguez, Roman;  ATMI LifeSciences
Claes, Tom;  ATMI LifeSciences
Moncaubeig, Fabien;  Artelis
Peeters, Ludovic;  GSK Vaccines
Crine, Michel ;  Université de Liège - ULiège > Département de chimie appliquée > Département de chimie appliquée
Toye, Dominique  ;  Université de Liège - ULiège > Département de chimie appliquée > Génie de la réaction et des réacteurs chimiques
Language :
English
Title :
HYDRODYNAMICS IN A DISPOSABLE RECTANGULAR PARALLELEPIPED STIRRED BIOREACTOR WITH ELLIPTIC PENDULUM MOTION PADDLE
Publication date :
15 January 2015
Journal title :
Biochemical Engineering Journal
ISSN :
1369-703X
Publisher :
Elsevier Science, Lausanne, Switzerland
Volume :
93
Peer reviewed :
Peer Reviewed verified by ORBi
Name of the research project :
Développement d'un modèle hydrodynamique multi-échelle décrivant le mélange dans les bioréacteurs utilisés en culture cellulaire. Validation expérimentale par les techniques de visualisation PIV et LIF.
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
Available on ORBi :
since 30 March 2015

Statistics


Number of views
108 (21 by ULiège)
Number of downloads
648 (10 by ULiège)

Scopus citations®
 
5
Scopus citations®
without self-citations
5
OpenCitations
 
5

Bibliography


Similar publications



Contact ORBi