Reference : Granulocyte chemotactic activity in exhaled breath condensate of healthy subjects and pa...
Scientific journals : Article
Human health sciences : Cardiovascular & respiratory systems
http://hdl.handle.net/2268/17927
Granulocyte chemotactic activity in exhaled breath condensate of healthy subjects and patients with COPD
English
Corhay, Jean-Louis mailto [Centre Hospitalier Universitaire de Liège - CHU > > Pneumologie-Allergologie >]
Hemelaers [ > > ]
Henket, Monique [Centre Hospitalier Universitaire de Liège - CHU > > Pneumologie-Allergologie >]
Sele, Jocelyne [Centre Hospitalier Universitaire de Liège - CHU > > Pneumologie-Allergologie >]
Louis, Renaud mailto [Université de Liège - ULg > Département des sciences cliniques > Pneumologie - Allergologie]
Jun-2007
CHEST
Amer Coll Chest Physicians
131
6
1672-1677
Yes (verified by ORBi)
International
0012-3692
Northbrook
[en] airway inflammation ; chemotactic activity ; COPD ; eosinophil ; exhaled breath condensate ; neutrophils
[en] Background. Several chemoattractants have been measured in exhaled breath condensate (EBC) from patients with COPD. The aim of this study was to compare the eosinophil. and neutrophil chemotactic activity contained in EBC from healthy subjects and patients with COPD. Methods: EBC collected using a commercially available condenser (EcoScreen; Erich Jaeger Viasys; Hoechberg, Germany) was compared in 45 COPD patients and 65 healthy subjects. EBC chemotactic activity for eosinophils and neutrophils was assessed using microchambers (Boyden; Neuro Probe; Cabin John, MD). Chemotactic index (CI) was used to evaluate cell migration. Results: EBC from patients with COPD (C1, 2.21 +/- 0.16 [mean +/- SEM]) and healthy subjects (CI, 1.67 +/- 0.11) displayed significant neutrophil chemotactic activity (p < 0.0001 for both), which was however higher in patients with COPD (p < 0.001). Healthy smokers had a significantly raised C1 for neutrophils by comparison with healthy nonsmokers (p < 0.01) and ex-smokers (p < 0.05). Ukewise, current COPD smokers tended to have greater neutrophil C1 than COPD who stopped smoking (p = 0.08). COPD ex-smokers had raised chemotactic activity by comparison with healthy ex-smokers (p < 0.05). Anti-interleuldn-8 (10(-6) g/mL) antibodies reduced neutrophil chemotactic activity by 35.2% (p < 0.05). EBC also contained significant eosinophil chemotactic activity in healthy subjects (CI, 1.68 0.09; p < 0.0001) and patients with COPD (CI, 1.23 +/- 0.07; p < 0.01), with a significantly lower CI in patients with COPD as compared to healthy subjects (p < 0.001). Smoking did not influence eosinophil chemotactic activity in healthy subjects or patients with COPD. Conclusions: Current smoking favors neutrophil chemotactic activity. As compared to healthy subjects, EBC from patients with COPD displays a skewed chemotactic activity toward neutrophils vs eosinophils.
http://hdl.handle.net/2268/17927
10.1378/chest.06-2225
http://www.chestjournal.org/content/131/6/1672.full.pdf+html

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