Article (Scientific journals)
Persistent infection of human thymic epithelial cells by coxsackievirus B4
Brilot, F.; Chehadeh, W.; Charlet-Renard, C. et al.
2002In Journal of Virology, 76 (10), p. 5260-5265
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Abstract :
[en] Persistent replication of coxsackievirus B4 (CVB4) E2 (diabetogenic) and CVB4 JBV (nondiabetogenic) strains in thymic epithelial cell (TEC)-enriched cultures (>or=95%) was proved by detection of positive- and negative-strand viral RNA by reverse transcription-PCR in extracted RNA from cell cultures, VP1 capsid protein detection by immunofluorescence (IF) staining, and release of infectious particles up to 30 days after infection without obvious cytolysis. By double-IF staining, cytokeratin-containing cells were shown to be susceptible to CVB4. The persistence of CVB4 was associated with a significantly increased rate of TEC proliferation (up to 70%) after 20 days of culture and a significantly increased chronic production of immunoreactive interleukin-6 (IL-6), leukemia inhibitory factor, and granulocyte-macrophage colony-stimulating factor in supernatant after 3 days of culture. The CVB4 replication and the release of cytokines were not restricted to the CVB4 E2 diabetogenic strain and did not depend on the genetic background of the host; however, TEC were more responsive to CVB4 E2 than CVB4 JBV as far as the production of cytokines.
Disciplines :
Immunology & infectious disease
Author, co-author :
Brilot, F.
Chehadeh, W.
Charlet-Renard, C.
Martens, Henri ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Embryologie
Geenen, Vincent ;  Université de Liège - ULiège > Centre d'immunologie
Hober, D.
Language :
English
Title :
Persistent infection of human thymic epithelial cells by coxsackievirus B4
Publication date :
May 2002
Journal title :
Journal of Virology
ISSN :
0022-538X
eISSN :
1098-5514
Publisher :
Amer Soc Microbiology, Washington, United States - Washington
Volume :
76
Issue :
10
Pages :
5260-5265
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 06 March 2015

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