ORBi: sbaa - caveolin plays a central role in endothelial progenitor cell mobilization and homing in SDF-1 driven postischemic vasculogenesis

Reference : caveolin plays a central role in endothelial progenitor cell mobilization and homing in ...


	Document type :	Scientific journals : Article
	Discipline(s) :	Human health sciences : Radiology, nuclear medicine & imaging Human health sciences : Oncology
	To cite this reference:	http://hdl.handle.net/2268/17885

Title :	caveolin plays a central role in endothelial progenitor cell mobilization and homing in SDF-1 driven postischemic vasculogenesis
Language :	English
Author, co-author :	sbaa [ > > ]
	MARTINIVE, Philippe [Université de Louvain > > > >]
	bouzin [ > > ]
	frerart [ > > ]
	balligand [ > > ]
	dessy [ > > ]
	feron [ > > ]
Other contributor :	dewever []
Publication date :	12-May-2006
Journal title :	Circulation Research
Publisher :	Lippincott Williams & Wilkins
Audience :	International
ISSN :	0009-7330
e-ISSN :	1524-4571
City :	Baltimore
Country :	MD
Keywords :	[en] caveolin ; endothelial progenitor ; SDF-1 vasculogenesis ; radiotherapy
Abstract :	[en] Caveolin plays a central role in endothelial progenitor cell mobilization and homing in SDF-1-driven postischemic vasculogenesis.Sbaa E, Dewever J, Martinive P, Bouzin C, Frérart F, Balligand JL, Dessy C, Feron O. Unit of Pharmacology and Therapeutics, University of Louvain Medical School, Brussels, Belgium. When neovascularization is triggered in ischemic tissues, angiogenesis but also (postnatal) vasculogenesis is induced, the latter requiring the mobilization of endothelial progenitor cells (EPC) from the bone marrow. Caveolin, the structural protein of caveolae, was recently reported to directly influence the angiogenic process through the regulation of the vascular endothelial growth factor (VEGF)/nitric oxide pathway. In this study, using caveolin-1 null mice (Cav(-/-)), we examined whether caveolin was also involved in the EPC recruitment in a model of ischemic hindlimb. Intravenous infusion of Sca-1(+) Lin(-) progenitor cells, but not bone marrow transplantation, rescued the defective neovascularization in Cav(-/-) mice, suggesting a defect in progenitor mobilization. The adhesion of Cav(-/-) EPC to bone marrow stromal cells indeed appeared to be resistant to the otherwise mobilizing SDF-1 (Stromal cell-Derived Factor-1) exposure because of a defect in the internalization of the SDF-1 cognate receptor CXCR4. Symmetrically, the attachment of Cav(-/-) EPC to SDF-1-presenting endothelial cells was significantly increased. Finally, EPC transduction with caveolin small interfering RNA reproduced this advantage in vitro and, importantly, led to a more extensive rescue of the ischemic hindlimb after intravenous infusion (versus sham-transfected EPC). These results underline the critical role of caveolin in ensuring the caveolae-mediated endocytosis of CXCR4, regulating both the SDF-1-mediated mobilization and peripheral homing of progenitor cells in response to ischemia. In particular, a transient reduction in caveolin expression was shown to therapeutically increase the engraftment of progenitor cells. PMID: 16601228 [PubMed - indexed for MEDLINE]
Target :	Researchers ; Professionals
Permalink :	http://hdl.handle.net/2268/17885

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