Article (Scientific journals)
What do Pneumocystis organisms tell us about the phylogeography of their hosts? The case of the woodmouse Apodemus sylvaticus in continental Europe and western Mediterranean islands
Demanche, christine; Deville, Manjula; Michaux, Johan et al.
2015In PLoS ONE, DOI:10.1371
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Keywords :
Pneumocystis, Apodemus sylvaticus, phylogeography, genetic diversity, phylogeny
Abstract :
[en] Pneumocystis fungi represent a highly diversified biological group with numerous species, which display a strong host-specificity suggesting a long co-speciation process. In the present study, the presence and genetic diversity of Pneumocystis organisms was investigated in 203 lung samples from woodmice (Apodemus sylvaticus) collected on western continental Europe and Mediterranean islands. The presence of Pneumocystis DNA was assessed by nested PCR at both large and small mitochondrial subunit (mtLSU and mtSSU) rRNA loci. Direct sequencing of nested PCR products demonstrated a very high variability among woodmouse-derived Pneumocystis organisms with a total number of 30 distinct combined mtLSU and mtSSU sequence types. However, the genetic divergence among these sequence types was very low (up to 3.87%) and the presence of several Pneumocystis species within Apodemus sylvaticus was considered unlikely. The analysis of the genetic structure of woodmouse-derived Pneumocystis revealed two distinct groups. The first one comprised Pneumocystis from woodmice collected in continental Spain, France and Balearic islands. The second one included Pneumocystis from woodmice collected in continental Italy, Corsica and Sicily. These two genetic groups were in accordance with the two lineages currently described within the host species Apodemus sylvaticus. Pneumocystis organisms are emerging as powerful tools for phylogeographic studies in mammals
Disciplines :
Zoology
Author, co-author :
Demanche, christine
Deville, Manjula
Michaux, Johan  ;  Université de Liège - ULiège > Département des sciences de la vie > Génétique et physiologie des microalgues
Barriel, Véronique
Pinçon, Claire
Aliouat-Denis, Cécile-Marie
Pottier, Muriel
Noël, Christophe
Viscoglios, Eric
Aliouat, El Moukhtar
Dei-Cas, Eduardo
Morand, Serge
Guillot, Jacques
More authors (3 more) Less
Language :
English
Title :
What do Pneumocystis organisms tell us about the phylogeography of their hosts? The case of the woodmouse Apodemus sylvaticus in continental Europe and western Mediterranean islands
Publication date :
2015
Journal title :
PLoS ONE
eISSN :
1932-6203
Publisher :
Public Library of Science, San Franscisco, United States - California
Volume :
DOI:10.1371
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 17 February 2015

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