Article (Scientific journals)
Increased of exhaled breath condensate neutrophil chemotaxis in acute exacerbation of COPD.
Corhay, Jean-Louis; MOERMANS, Catherine; HENKET, Monique et al.
2014In Respiratory Research, 15, p. 115
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Abstract :
[en] BACKGROUND: Neutrophils have been involved in the pathogenesis of chronic obstructive pulmonary disease (COPD). Underlying mechanisms of neutrophil accumulation in the airways of stable and exacerbated COPD patients are poorly understood. The aim of this study was to assess exhaled breath condensate (EBC) neutrophil chemotactic activity, the level of two chemoattractants for neutrophils (GRO-alpha and LTB4) during the course of an acute exacerbation of COPD (AECOPD). METHODS: 50 ex smoking COPD patients (33 with acute exacerbation and 17 in stable disease) and 20 matched ex smoking healthy controls were compared. EBC was collected by using a commercially available condenser (EcoScreen(R)). EBC neutrophil chemotactic activity (NCA) was assessed by using Boyden microchambers. Chemotactic index (CI) was used to evaluate cell migration. LTB4 and GROalpha levels were measured by a specific enzyme immunoassay in EBC. RESULTS: Stable COPD and outpatients with AECOPD, but not hospitalized with AECOPD, had raised EBC NCA compared to healthy subjects (p < 0.05 and p < 0.01 respectively). In outpatients with AECOPD EBC NCA significantly decreased 6 weeks after the exacerbation. Overall EBC NCA was weakly correlated with sputum neutrophil counts (r = 0.26, p < 0.05). CONCLUSIONS: EBC NCA rose during acute exacerbation of COPD in ambulatory patients and decreased at recovery. While LTB4 seems to play a role both in stable and in exacerbated phase of the disease, the role of GRO-alpha as a chemotactic factor during AECOPD is not clearly established and needs further investigation.
Disciplines :
Cardiovascular & respiratory systems
Author, co-author :
Corhay, Jean-Louis ;  Université de Liège - ULiège > Département des sciences cliniques > Département des sciences cliniques
MOERMANS, Catherine  ;  Centre Hospitalier Universitaire de Liège - CHU > Pneumologie-Allergologie
HENKET, Monique ;  Centre Hospitalier Universitaire de Liège - CHU > Pneumologie-Allergologie
NGUYEN DANG, Delphine ;  Centre Hospitalier Universitaire de Liège - CHU > Pneumologie-Allergologie
DUYSINX, Bernard ;  Centre Hospitalier Universitaire de Liège - CHU > Pneumologie-Allergologie
Louis, Renaud ;  Université de Liège - ULiège > Département des sciences cliniques > Pneumologie - Allergologie
Language :
English
Title :
Increased of exhaled breath condensate neutrophil chemotaxis in acute exacerbation of COPD.
Publication date :
28 September 2014
Journal title :
Respiratory Research
ISSN :
1465-9921
eISSN :
1465-993X
Publisher :
BioMed Central, United Kingdom
Volume :
15
Pages :
115
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 16 February 2015

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