Article (Scientific journals)
Sequential Autoprocessing of Marek’s Disease Herpesvirus Protease Differs from That of Other Herpesviruses
Laurent, Sylvie; Blondeau, Caroline; Belghazi, Maya et al.
2007In Journal of Virology
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Keywords :
Marek's disease virus; serine protease; R and M sites
Abstract :
[en] Herpesviruses encode a unique serine protease essential for viral capsid maturation. This protease undergoes autoprocessing at two sites, R and M, at the consensus sequence (V, L,I)P3-XP2-AP1/SP1' (where X is a polar amino acid). We observed complete autoprocessing at the R and M sites of Marek’s disease virus (MDV) protease following production of the polyprotein in Escherichia coli. Site-directed mutagenesis confirmed the predicted sequence of the R and M sites, with the M site sequence being nonconsensual: MP3-NP2-AP1/SP1'. Mutagenesis and expression kinetics studies suggested that the atypical MDV M site was cleaved exclusively by the processed short protease, a feature making MDV unique among herpesviruses.
Disciplines :
Life sciences: Multidisciplinary, general & others
Author, co-author :
Laurent, Sylvie;  Institut Scientifique de Recherche Agronomique - INRA > sante animale > Equipe Telomerase et Lymphome Viro-induit, UPR INRA 1282 IASP-213, Nouzilly
Blondeau, Caroline ;  Institut Scientifique de Recherche Agronomique - INRA > Sante Animale > Laboratoire de Virologie Moleculaire, UR1282, IASP, Nouzilly
Belghazi, Maya;  Institut Scientifique de Recherche Agronomique - INRA > Service de Spectrometrie de Masse pour la proteomique, Unite PRC, Nouzilly
Remy, Sylvie;  Institut Scientifique de Recherche Agronomique - INRA > Sante Animale > Equipe Telomerase et Lymphome Viro-induit, UPR INRA 1282 IASP-213, Nouzilly
Esnault, Evelyne;  Institut Scientifique de Recherche Agronomique - INRA > Sante Animale > Equipe Telomerase et Lymphome Viro-induit, UPR INRA 1282 IASP-213, Nouzilly
Rasschaert, P;  Institut Scientifique de Recherche Agronomique - INRA > Sante Animale > Equipe Telomerase et Lymphome Viro-induit, UPR INRA 1282 IASP-213, Nouzilly
Rasschaert, Denis;  Institut Scientifique de Recherche Agronomique - INRA > Sante Animale > Equipe Telomerase et Lymphome Viro-induit, UPR INRA 1282 IASP-213, Nouzilly
Language :
English
Title :
Sequential Autoprocessing of Marek’s Disease Herpesvirus Protease Differs from That of Other Herpesviruses
Publication date :
2007
Journal title :
Journal of Virology
ISSN :
0022-538X
eISSN :
1098-5514
Publisher :
American Society for Microbiology (ASM), Washington, United States - District of Columbia
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 11 February 2015

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