Article (Scientific journals)
Characterisation of polymorphic microsatellite loci in Hephaestus fuliginosus and cross-amplification in closely related Hephaestus tulliensis
Jamandre, Brian Wade; Real, K.; Hughes, J.
2012In Conservation Genetics Resources, 4 (2), p. 213-216
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Keywords :
Freshwater fish; Grunters; Northern Australia; Population genetic analysis
Abstract :
[en] Eleven polymorphic microsatellite loci were isolated and characterised from sooty grunter (Hephaestus fuliginosus) and Tully grunter (H. tulliensis) from Northern Australia. Each primer pair amplified good quality and polymorphic products. The versatilities of these markers were tested using different H. fuliginosus populations and cross-amplified to a closely related species, H. tulliensis, found within the northern Australian wet tropics. The number of alleles ranged from 5 to 20 per locus and expected heterozygosities ranged from 0.043 to 0.926. All loci conformed to Hardy-Weinberg expectations, with the exception of 2Hf44 and 2Hf53 for Daly R. and Mitchell R. populations of H. fuliginosus, respectively. No evidence of linkage disequilibrium was detected between any pair of loci. The markers reported here would be very useful for population genetic studies, evolution and conservation of these species. © 2011 Springer Science+Business Media B.V.
Disciplines :
Zoology
Author, co-author :
Jamandre, Brian Wade ;  Université de Liège - ULiège > Département de Biologie, Ecologie et Evolution > Biologie du comportement - Ethologie et psychologie animale
Real, K.;  Australian Rivers Institute, Griffith University, 170 Kessels Rd., Nathan, QLD, 4111, Australia
Hughes, J.;  Australian Rivers Institute, Griffith University, 170 Kessels Rd., Nathan, QLD, 4111, Australia
Title :
Characterisation of polymorphic microsatellite loci in Hephaestus fuliginosus and cross-amplification in closely related Hephaestus tulliensis
Publication date :
2012
Journal title :
Conservation Genetics Resources
ISSN :
1877-7252
eISSN :
1877-7260
Publisher :
Springer, Germany
Volume :
4
Issue :
2
Pages :
213-216
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 10 February 2015

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