[en] The transcription start point of the dnaK operon of Streptomyces coelicolor A3(2) has been determined by S1 mapping, using the EMBL automated fluorescent DNA sequencer. The -35 and -10 hexamers correspond to a sigma 70-type promoter. This promoter responds to heat shock and involves an inverted repeat different from the CIRCE sequence characteristic of the Gram-positive heat-shock promoters.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Brans, Alain ; Université de Liège - ULiège > Centre d'ingénierie des protéines
Loriaux, Axelle; Université de Liège - ULiège > Centre d'ingénierie des protéines
Thamm, Iris ; Université de Liège - ULiège > Centre d'ingénierie des protéines
Joris, Bernard ; Université de Liège - ULiège > Centre d'ingénierie des protéines
Dusart, Jean; Université de Liège - ULiège > Centre d'Ingénierie des Protéines
Language :
English
Title :
Use of an Automatic DNA Sequencer for S1 Mapping: Transcriptional Analysis of the Streptomyces Coelicolor A3(2) Dnak Operon
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