Article (Scientific journals)
Morphological and molecular characterization of planktonic cyanobacteria from Belgium and Luxembourg
Willame, R.; Boutte, C.; Grubisic, Stana et al.
2006In Journal of Phycology, 42 (6), p. 1312-1332
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Keywords :
16S rRNA gene; bloom; freshwater cyanobacteria; nanoplankton; periphyton; phytobenthos; picoplankton; polyphasic taxonomy
Abstract :
[en] For the first time in Belgium and Luxembourg, the diversity and taxonomy of 95 cyanobacterial strains isolated from freshwater blooms were assessed by the comparison of phenotypes and partial 16S rRNA gene sequences. The results showed the high diversity of nanoplanktonic, picoplanktonic, and benthic-periphytic cyanobacteria accompanying the main bloom-forming taxa. Indeed, besides 15 morphotypes of bloom-forming taxa, seven non-bloom-forming planktonic morphotypes and 11 morphotypes from benthic-periphytic taxa were isolated in culture from the plankton samples of 35 water bodies. The bloom-forming strains belonged to the genera Microcystis, Woronichinia, Planktothrix, Anabaena, and Aphanizomenon, whereas the other strains isolated from the same samples were assigned to the nanoplanktonic Aphanocapsa, Aphanothece, Snowella, and Pseudanabaena; to the picoplanktonic Cyanobium; and to the benthic periphytic Geitlerinema, Komvophoron, Leptolyngbya, Lyngbya, Phormidium, Calothrix, Nostoc, and Trichormus. The results supported both the polyphyletism of genera such as Aphanocapsa, Aphanothece, Leptolyngbya, Geitlerinema, Anabaena, and Aphanizomenon as well as the validity of genera such as Microcystis, Planktothrix, and Pseudanabaena with gas vesicles and cells constricted at the cross wall. The results obtained showed the close relationship between Snowella and Woronichinia for which very few sequences exist. The first sequence of Komvophoron appeared poorly related to other available cyanobacterial sequences. Although in a few cases a good agreement existed between phenotypic and genotypic features, there was generally a discrepancy. Strains with identical morphotypes show small differences in the 16S rRNA sequences, which might be related to the different chemical properties of their habitats. The results showed the importance of the polyphasic approach in order to improve the taxonomy of cyanobacteria.
Disciplines :
Aquatic sciences & oceanology
Phytobiology (plant sciences, forestry, mycology...)
Author, co-author :
Willame, R.
Boutte, C.
Grubisic, Stana ;  Université de Liège - ULiège > Services administratifs généraux > Protection et hygiène du travail (SUPHT)
Wilmotte, Annick  ;  Université de Liège - ULiège > Département des sciences de la vie > Enzymologie
Komarek, J.
Hoffmann, L.
Language :
English
Title :
Morphological and molecular characterization of planktonic cyanobacteria from Belgium and Luxembourg
Publication date :
December 2006
Journal title :
Journal of Phycology
ISSN :
0022-3646
eISSN :
1529-8817
Publisher :
Blackwell Publishing, Oxford, United Kingdom
Volume :
42
Issue :
6
Pages :
1312-1332
Peer reviewed :
Peer Reviewed verified by ORBi
Name of the research project :
MIDI-CHIP
Funders :
UE - Union Européenne [BE]
Available on ORBi :
since 03 August 2009

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