Article (Scientific journals)
Le dosage de la vitamine D: considérations pré-analytiques et analytiques
LE GOFF, Caroline; Souberbielle, Jean-Claude; Delvin, Edgard et al.
2015In Annales de Biologie Clinique, 73 (1), p. 79-92
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Keywords :
vitamin D; cholecalciferol; ergocalciferol; 25(OH)D; mass spectrometry; immunoassay
Abstract :
[en] The constantly increasing requests for the measurement of serum 25-hydroxyvitamin D over the last years has led reagent manufacturers to market different automated and semi-automated methods, that being unfortunately not fully harmonized, yield different results. Liquid chromatography coupled to tandem mass spectrometry has more recently been introduced. This approach allows the distinction between the two forms of 25-hydroxyvitamin D and to measure other metabolites. This approach also requires harmonization to curtail the differences between the different analytical methods. To meet this requirement, the American national institutes of health (NIH), the CDC (Center for disease control and prevention) in Atlanta, the NIST (National institute of standards and technology) and the vitamin D Reference laboratory of Ghent University have pooled their expertise to develop a standardization program. This article reviews the main elements and the difficulties of the automated and semi-automated methods for 25-hydroxyvitamin D, from sample preparation to the analytical phase, as well as those related to mass spectrometry. It also addresses the issues related to the clinical decision thresholds and the possibility of measurements in different biological liquids.
Disciplines :
Laboratory medicine & medical technology
Author, co-author :
LE GOFF, Caroline  ;  Centre Hospitalier Universitaire de Liège - CHU > Chimie médicale
Souberbielle, Jean-Claude
Delvin, Edgard
CAVALIER, Etienne  ;  Centre Hospitalier Universitaire de Liège - CHU > Chimie médicale
Language :
French
Title :
Le dosage de la vitamine D: considérations pré-analytiques et analytiques
Alternative titles :
[en] Vitamin D measurement: pre-analytical an analytical considerations
Publication date :
February 2015
Journal title :
Annales de Biologie Clinique
ISSN :
0003-3898
eISSN :
1950-6112
Publisher :
John Libbey Eurotext, Montrouge, France
Volume :
73
Issue :
1
Pages :
79-92
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 19 January 2015

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