Article (Scientific journals)
Evaluation of growth/no growth interface of Listeria monocytogenes growing on stainless steel surfaces, detached from biofilms or in suspension, in response to pH and NaCl.
Belessi, Charalambia-Eirini A.; Gounadaki, Antonia S.; Schvartzman Echenique, Maria Sol et al.
2011In International Journal of Food Microbiology, 145 Suppl 1, p. 53-60
Peer Reviewed verified by ORBi
 

Files


Full Text
Belessi2011IJFM.pdf
Publisher postprint (731.93 kB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
Biofilms/growth & development; Food Microbiology/methods; Hydrogen-Ion Concentration; Listeria monocytogenes/drug effects/growth & development/physiology; Sodium Chloride/pharmacology; Stainless Steel; Suspensions
Abstract :
[en] The present study aimed to describe the growth/no growth interface of Listeria monocytogenes at three potential states of growth in industrial environments, namely attached, (Att), detached (Det) from a biofilm, or in a planktonic state (suspended; Plan). A 3-strain composite of L. monocytogenes cells was left to colonize stainless steel (SS) surfaces in tryptic soy broth supplemented with 0.6% yeast extract (TSBYE) at 20 degrees C for 72 h and then transferred to TSBYE at 30 different pH and NaCl concentrations, which were renewed every two days during incubation at 10 degrees C. Survival of attached population was observed at optimal conditions (pH 7.2, a(w) 0.996), whereas at 4.5-8.0% salt and/or pH<6.0, reduction of attached population on SS surfaces was observed. PFGE patterns showed that 91% of the cells colonizing the SS coupons after 30 days, at any pH and a(w) conditions, belonged to a single strain. Furthermore, the change in the probability of a single cell to initiate growth (P(in)) over time, as well as the number of cells needed (CN) for growth initiation of planktonically growing Plan and Det L. monocytogenes cells were evaluated based on MPN Tables. An ordinary logistic regression model was also used to describe the growth/no growth interface of varying inoculation levels (from <10 to 10(4)CFU/ml) of Plan and Det cells in response to pH and a(w). Although both cell types demonstrated similar growth limits at populations of 10(2)-10(4)CFU/ml, higher numbers of Det than Plan cells were needed (CN) in order to initiate growth at low a(w) and pH. Individual Plan cells reached higher maximum levels of probability of growth initiation (P(max)) and had shorter times to reach P(max)/2 (t(au)), compared to their Det counterparts. Data on growth potential of cells in suspension, attached or detached status, may assist in ranking the risk from different sources of contamination. In addition, they may establish the link between the behavior of L. monocytogenes in foods and its origin from the processing plant. The latter link is important component of biotraceability.
Disciplines :
Food science
Author, co-author :
Belessi, Charalambia-Eirini A.
Gounadaki, Antonia S.
Schvartzman Echenique, Maria Sol ;  Université de Liège - ULiège > Département des sciences de la vie > Génomique fonctionnelle et imagerie moléculaire végétale
Jordan, Kieran
Skandamis, Panagiotis N.
Language :
English
Title :
Evaluation of growth/no growth interface of Listeria monocytogenes growing on stainless steel surfaces, detached from biofilms or in suspension, in response to pH and NaCl.
Publication date :
2011
Journal title :
International Journal of Food Microbiology
ISSN :
0168-1605
eISSN :
1879-3460
Publisher :
Elsevier, Netherlands
Volume :
145 Suppl 1
Pages :
S53-60
Peer reviewed :
Peer Reviewed verified by ORBi
Commentary :
Copyright (c) 2010 Elsevier B.V. All rights reserved.
Available on ORBi :
since 14 January 2015

Statistics


Number of views
58 (4 by ULiège)
Number of downloads
2 (2 by ULiège)

Scopus citations®
 
13
Scopus citations®
without self-citations
10
OpenCitations
 
14

Bibliography


Similar publications



Contact ORBi