Article (Scientific journals)
Low-Molecular-Weight Protein Tyrosine Phosphatases of Bacillus subtilis
Musumeci, Lucia; Bongiorni, Cristina; Tautz, Lutz et al.
2005In Journal of Bacteriology, 187 (14), p. 4945-4956
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Keywords :
LMPTP; Bacillus subtilis; ethanol stress
Abstract :
[en] In gram-negative organisms, enzymes belonging to the low-molecular-weight protein tyrosine phosphatase (LMPTP) family are involved in the regulation of important physiological functions, including stress resistance and synthesis of the polysaccharide capsule. LMPTPs have been identified also in gram-positive bacteria, but their functions in these organisms are presently unknown. We cloned two putative LMPTPs from Bacillus subtilis, YfkJ and YwlE, which are highly similar to each other in primary structure as well as to LMPTPs from gram-negative bacteria. When purified from overexpressing Escherichia coli strains, both enzymes were able to dephosphorylate p-nitrophenyl-phosphate and phosphotyrosine-containing substrates in vitro but showed significant differences in kinetic parameters and sensitivity to inhibitors. Transcriptional analyses showed that yfkJ was transcribed at a low level throughout the growth cycle and underwent a σB-dependent transcriptional upregulation in response to ethanol stress. The transcription of ywlE was growth dependent but stress insensitive. Genomic deletion of each phosphatase-encoding gene led to a phenotype of reduced bacterial resistance to ethanol stress, which was more marked in the ywlE deletion strain. Our study suggests that YfkJ and YwlE play roles in B. subtilis stress resistance.
Research center :
Inflammatory and Infectious Diseases Center, The Burnham Institute
Division of Cellular Biology, Department of Molecular and Experimental Medicine, The Scripps Research Institute
Fellowship for the Interpretation of Genomes, Center for Microbial Sciences, San Diego State University
Disciplines :
Microbiology
Author, co-author :
Musumeci, Lucia ;  Université de Liège - ULiège > GIGA-Research
Bongiorni, Cristina
Tautz, Lutz
Edwards, Robert
Osterman, Andrei
Perego, Marta
Mustelin, Tomas
Bottini, Nunzio
Language :
English
Title :
Low-Molecular-Weight Protein Tyrosine Phosphatases of Bacillus subtilis
Publication date :
July 2005
Journal title :
Journal of Bacteriology
ISSN :
0021-9193
eISSN :
1098-5530
Publisher :
American Society for Microbiology (ASM), Washington, United States - District of Columbia
Volume :
187
Issue :
14
Pages :
4945-4956
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
NIH - National Institutes of Health [US-MD] [US-MD]
Danish National Research Council - SNF
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since 04 January 2015

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