Article (Scientific journals)
Rac1 GTPase silencing counteracts microgravity-induced effects on osteoblastic cells.
Guignandon, Alain; Fauré, Céline; Neutelings, Thibaut et al.
2014In FASEB Journal, 28 (9), p. 4077-4087
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Keywords :
microgravity; RhoGTPases; osteoblast
Abstract :
[en] Bone cells exposed to real microgravity display alterations of their cytoskeleton and focal adhesions, two major mechanosensitive structures. These structures are controlled by small GTPases of the Ras homology (Rho) family. We investigated the effects of RhoA, Rac1, and Cdc42 modulation of osteoblastic cells under microgravity conditions. Human MG-63 osteoblast-like cells silenced for RhoGTPases were cultured in the automated Biobox bioreactor (European Space Agency) aboard the Foton M3 satellite and compared to replicate ground-based controls. The cells were fixed after 69 h of microgravity exposure for postflight analysis of focal contacts, F-actin polymerization, vascular endothelial growth factor (VEGF) expression, and matrix targeting. We found that RhoA silencing did not affect sensitivity to microgravity but that Rac1 and, to a lesser extent, Cdc42 abrogation was particularly efficient in counteracting the spaceflight-related reduction of the number of focal contacts [-50% in silenced, scrambled (SiScr) controls vs. -15% for SiRac1], the number of F-actin fibers (-60% in SiScr controls vs. -10% for SiRac1), and the depletion of matrix-bound VEGF (-40% in SiScr controls vs. -8% for SiRac1). Collectively, these data point out the role of the VEGF/Rho GTPase axis in mechanosensing and validate Rac1-mediated signaling pathways as potential targets for counteracting microgravity effects
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Guignandon, Alain
Fauré, Céline
Neutelings, Thibaut ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Laboratoire de Biologie des Tissus conjonctifs
Rattner, Aline
Mineur, Pierre
Linossier, Marie-Thérèse
Laroche, Norbert
Lambert, Charles ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Laboratoire des tissus conjonctifs
Deroanne, Christophe ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Laboratoire des tissus conjonctifs
Nusgens, Betty ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques
Demets, René
Colige, Alain ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Laboratoire des tissus conjonctifs
Vico, Laurence
More authors (3 more) Less
Language :
English
Title :
Rac1 GTPase silencing counteracts microgravity-induced effects on osteoblastic cells.
Publication date :
September 2014
Journal title :
FASEB Journal
ISSN :
0892-6638
eISSN :
1530-6860
Publisher :
Federation of American Society for Experimental Biology, Bethesda, United States - Maryland
Volume :
28
Issue :
9
Pages :
4077-4087
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 20 November 2014

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