Article (Scientific journals)
Micelle size characterization of lipopeptides produced by B. subtilis and their recovery by the two-step ultrafiltration process.
JAUREGI, P.; COUTTE, F.; CATIAU, L. et al.
2013In Separation and Purification Technology, 104, p. 175-182
Peer Reviewed verified by ORBi
 

Files


Full Text
Art 84 (2013).pdf
Publisher postprint (450.73 kB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
Ultrafiltration; Membrane purification; Lipopeptide; B. subtilis; Micelles
Abstract :
[en] The aim of this work was to investigate the lipopeptides aggregation behavior in single and mixed solutions in a wide range of concentrations, in order to optimize their separation and purification following the two-step ultrafiltration process and using large pore size membranes (up to MWCO = 300 kDa). Micelle size was determined by dynamic light scattering. In single solutions of lipopeptide both surfactin and mycosubtilin formed micelles of different size depending on their concentration, micelles of average diameter = 5–105 nm for surfactin and 8–18 nm for mycosubtilin. However when the lipopeptides were in the same solution they formed mixed micelles of different size (d = 8 nm) and probably conformation to that formed by the individual lipopeptide, this prevents their separation according to size. These lipopeptides were purified from fermentation culture by the two-step ultrafiltration process using different MWCO membranes ranging from 10 to 300 kDa. This led to their effective rejection in the first ultrafiltration step by membranes with MCWO = 10–100 kDa but poor rejection by the 300 KDa membrane. The lipopeptides were recovered at 90% purity (in relation to protein) and with 2.34 enrichment in the permeate of the second ultrafiltration step with the 100 KDa membrane upon addition of 75% ethanol
Disciplines :
Microbiology
Author, co-author :
JAUREGI, P.
COUTTE, F.
CATIAU, L.
LECOUTURIER, D.
Jacques, Philippe ;  Université de Liège - ULiège > Chimie et bio-industries > Bio-industries
Language :
English
Title :
Micelle size characterization of lipopeptides produced by B. subtilis and their recovery by the two-step ultrafiltration process.
Publication date :
2013
Journal title :
Separation and Purification Technology
ISSN :
1383-5866
eISSN :
1873-3794
Publisher :
Elsevier Science
Volume :
104
Pages :
175-182
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 04 November 2014

Statistics


Number of views
61 (2 by ULiège)
Number of downloads
0 (0 by ULiège)

Scopus citations®
 
46
Scopus citations®
without self-citations
31
OpenCitations
 
42

Bibliography


Similar publications



Contact ORBi