Article (Scientific journals)
One-pot enzymatic synthesis of the Gal alpha 1-->3Gal beta 1-->4GlcNAc sequence with in situ UDP-Gal regeneration.
Hokke, Cornelis H; Zervosen, Astrid; Elling, Lothar et al.
1996In Glycoconjugate Journal, 13 (4), p. 687-92
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Keywords :
Animals; Carbohydrate Sequence; Cattle; Enzyme Stability; Galactosyltransferases/chemistry; Glucosyltransferases/chemistry; Kinetics; Magnetic Resonance Spectroscopy; Molecular Sequence Data; Oligosaccharides/chemical synthesis; UDPglucose 4-Epimerase/chemistry; Uridine Diphosphate Galactose/chemistry
Abstract :
[en] The trisaccharide Gal alpha 1-->3Gal beta 1-->4GlcNAc beta 1-->O-(CH2)8COOCH3 was enzymatically synthesized, with in situ UDP-Gal regeneration. By combination in one pot of only four enzymes, namely, sucrose synthase, UDP-Glc 4'-epimerase, UDP-Gal:GlcNAc beta 4-galactosyltransferase and UDP-Gal:Gal beta 1-->4GlcNAc alpha 3-galactosyltransferase, Gal alpha 1-->3Gal beta 1-->4GlcNAc beta 1-->O-(CH2)8COOCH3 was formed in a 2.2 mumol ml-1 yield starting from the acceptor GlcNAc beta 1-->O-(CH2)8COOCH3. This is an efficient and convenient method for the synthesis of the Gal alpha 1-->3Gal beta 1-->4GlcNAc epitope which pays an important role in various biological and immunological processes.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Hokke, Cornelis H
Zervosen, Astrid ;  Université de Liège - ULiège > Centre de recherches du cyclotron
Elling, Lothar
Joziasse, David H.
van den Eijnden, Dirk H.
Language :
English
Title :
One-pot enzymatic synthesis of the Gal alpha 1-->3Gal beta 1-->4GlcNAc sequence with in situ UDP-Gal regeneration.
Publication date :
1996
Journal title :
Glycoconjugate Journal
ISSN :
0282-0080
eISSN :
1573-4986
Publisher :
Kluwer Academic Publishers, Boston, United States - Massachusetts
Volume :
13
Issue :
4
Pages :
687-92
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 23 July 2009

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