Reference : One-pot enzymatic synthesis of the Gal alpha 1-->3Gal beta 1-->4GlcNAc sequence with ...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/17345
One-pot enzymatic synthesis of the Gal alpha 1-->3Gal beta 1-->4GlcNAc sequence with in situ UDP-Gal regeneration.
English
Hokke, Cornelis H [> > > >]
Zervosen, Astrid mailto [Université de Liège - ULg > > Centre de recherches du cyclotron >]
Elling, Lothar [> > > >]
Joziasse, David H. [> > > >]
van den Eijnden, Dirk H. [> > > >]
1996
Glycoconjugate Journal
Kluwer Academic Publishers
13
4
687-92
Yes (verified by ORBi)
International
0282-0080
Boston
MA
[en] Animals ; Carbohydrate Sequence ; Cattle ; Enzyme Stability ; Galactosyltransferases/chemistry ; Glucosyltransferases/chemistry ; Kinetics ; Magnetic Resonance Spectroscopy ; Molecular Sequence Data ; Oligosaccharides/chemical synthesis ; UDPglucose 4-Epimerase/chemistry ; Uridine Diphosphate Galactose/chemistry
[en] The trisaccharide Gal alpha 1-->3Gal beta 1-->4GlcNAc beta 1-->O-(CH2)8COOCH3 was enzymatically synthesized, with in situ UDP-Gal regeneration. By combination in one pot of only four enzymes, namely, sucrose synthase, UDP-Glc 4'-epimerase, UDP-Gal:GlcNAc beta 4-galactosyltransferase and UDP-Gal:Gal beta 1-->4GlcNAc alpha 3-galactosyltransferase, Gal alpha 1-->3Gal beta 1-->4GlcNAc beta 1-->O-(CH2)8COOCH3 was formed in a 2.2 mumol ml-1 yield starting from the acceptor GlcNAc beta 1-->O-(CH2)8COOCH3. This is an efficient and convenient method for the synthesis of the Gal alpha 1-->3Gal beta 1-->4GlcNAc epitope which pays an important role in various biological and immunological processes.
http://hdl.handle.net/2268/17345
10.1007/BF00731458

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