Application of recombinant sucrose synthase large scale synthesis of ADP-glucose

Abstract
The synthesis of ADP-glucose with recombinant sucrose synthase from potato was combined with the synthesis of ADP from AMP and ATP catalysed by myokinase from rabbit muscle. By using the repetitive-batch-technique we were able to reach enzyme productivities (mg ADP-glucose/U enzyme) of 28 mg/U sucrose synthase and 140 mg/U myokinase yielding 2.8 g ADP-glucose (55% yield). After product isolation, 2.2 g ADP-glucose was obtained corresponding to 44% overall yield.