Reference : Application of recombinant sucrose synthase large scale synthesis of ADP-glucose
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/17342
Application of recombinant sucrose synthase large scale synthesis of ADP-glucose
English
Zervosen, Astrid mailto [Université de Liège - ULg > > Centre de recherches du cyclotron >]
Romer, Ulrike [> > > >]
Elling, Lothar [> > > >]
1998
Journal of Molecular Catalysis B : Enzymatic
Elsevier Science
5
1-4
Sp. Iss. SI
25-28
Yes (verified by ORBi)
International
1381-1177
Amsterdam
The Netherlands
[en] ADP-Glucose ; sucrose synthase ; nucleotide sugar
[en] Abstract
The synthesis of ADP-glucose with recombinant sucrose synthase from potato was combined with the synthesis of ADP from AMP and ATP catalysed by myokinase from rabbit muscle. By using the repetitive-batch-technique we were able to reach enzyme productivities (mg ADP-glucose/U enzyme) of 28 mg/U sucrose synthase and 140 mg/U myokinase yielding 2.8 g ADP-glucose (55% yield). After product isolation, 2.2 g ADP-glucose was obtained corresponding to 44% overall yield.
http://hdl.handle.net/2268/17342
10.1016/S1381-1177(98)00040-X

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