ORBi: Zervosen Astrid - Characterization of the cattle serum antibody responses against TEM beta-lactamase and the nonimmunogenic Escherichia coli heat-stable enterotoxin (STaI)

Reference : Characterization of the cattle serum antibody responses against TEM beta-lactamase and t...


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Biochemistry, biophysics & molecular biology Life sciences : Veterinary medicine & animal health
	To cite this reference:	http://hdl.handle.net/2268/17340

Title :	Characterization of the cattle serum antibody responses against TEM beta-lactamase and the nonimmunogenic Escherichia coli heat-stable enterotoxin (STaI)
Language :	English
Author, co-author :	Zervosen, Astrid [Université de Liège - ULg > > Centre de recherches du cyclotron >]
	Saegerman, Claude [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Epidémiologie et analyse des risques appl. aux sc. vétér. >]
	Antoniotti, Ingrid [> > > >]
	Robert, Béatrice [> > > >]
	Ruth, Nadia [> > > >]
	Collard, Alfred [> >]
	Schynts, Frédéric [> > > >]
	Galleni, Moreno [Université de Liège - ULg > Département des sciences de la vie > Macromolécules biologiques >]
	Mercuri, Paola [Université de Liège - ULg > > Centre d'ingénierie des protéines >]
Publication date :	2008
Journal title :	FEMS Immunology & Medical Microbiology
Publisher :	Blackwell Publishing
Volume :	54
Issue/season :	3
Pages :	319-329
Audience :	International
ISSN :	0928-8244
City :	Oxford
Country :	United Kingdom
Keywords :	[en] heat-stable enterotoxin STa ; beta-lactamase TEM-1 ; vaccine ; cattle ; hybrid protein
Abstract :	[en] In order to test the use of a subunit recombinant vaccine for its capacity to induce antibodies against the nonimmunogenic heat-stable enterotoxin STa from Escherichia coli and the TEM-1 beta-lactamase, cattle were immunized with a hybrid protein created by insertion of the STa sequence at position 197 of the TEM-1 beta-lactamase. Specific anti-STa IgG and IgG1 antibodies were detected at low levels, while no IgG2 antibodies were detected. In contrast, high levels of the different anti-TEM IgG subtypes were detected in cattle sera. In addition, beta-lactamase activity was inhibited by the sera. The presence of antibodies against STa and TEM-1 beta-lactamase was assessed in sera from 366 cattle taken from the field. No significant level of IgGs against the toxin or the TEM-1 was detected. A comparison of the antibody level between the immunized and the nonimmunized animals clearly demonstrated that STa was not able to induce a significant level of antibodies in the vaccinated animals. In contrast, a strong antibody response against TEM-1 beta-lactamase was demonstrated.
Research units :	Centre d'Ingénierie des Protéines - CIP
Permalink :	http://hdl.handle.net/2268/17340
DOI :	10.1111/j.1574-695X.2008.00482.x

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