Article (Scientific journals)
Resistance to cassava mosaic disease in transgenic cassava expressing antisense RNAs targeting virus replication genes.
Zhang, Peng; Vanderschuren, Hervé; Futterer, Johannes et al.
2005In Plant Biotechnology Journal, 3 (4), p. 385-97
Peer Reviewed verified by ORBi
 

Files


Full Text
Zhangetal2005.pdf
Publisher postprint (447.91 kB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Abstract :
[en] African cassava mosaic virus (ACMV) is a major contributor to cassava mosaic disease (CMD), the economically most important and devastating disease of cassava in Africa. We have developed transgenic cassava plants with increased ACMV resistance using improved antisense RNA technology by targeting the viral mRNAs of Rep (AC1), TrAP (AC2) and REn (AC3). Viral DNA replication assays in detached leaves demonstrated that replication of two ACMV isolates was strongly reduced or inhibited in most transgenic lines. After ACMV infection of plants using biolistic inoculation, several lines remained symptomless at lower infection pressure (100 ng viral DNA/plant). Symptom development was reduced and attenuated even at higher DNA doses. Transgenic ACMV-resistant plants had significantly reduced viral DNA accumulation in their infected leaves. Short sense and antisense RNAs specific to AC1 were identified in transgenic lines expressing AC1 antisense RNA, suggesting that the short RNAs mediate interference by post-transcriptional gene silencing. Our results demonstrate that resistance to ACMV infection of cassava can be achieved with high efficacy by expressing antisense RNAs against viral mRNAs encoding essential non-structural proteins, providing a new tool to combat CMD in Africa.
Disciplines :
Biotechnology
Author, co-author :
Zhang, Peng
Vanderschuren, Hervé  ;  Université de Liège - ULiège > Sciences agronomiques > Biologie végétale
Futterer, Johannes
Gruissem, Wilhelm
Language :
English
Title :
Resistance to cassava mosaic disease in transgenic cassava expressing antisense RNAs targeting virus replication genes.
Publication date :
2005
Journal title :
Plant Biotechnology Journal
ISSN :
1467-7644
eISSN :
1467-7652
Publisher :
Blackwell, Oxford, United Kingdom
Volume :
3
Issue :
4
Pages :
385-97
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 06 October 2014

Statistics


Number of views
38 (2 by ULiège)
Number of downloads
0 (0 by ULiège)

Scopus citations®
 
101
Scopus citations®
without self-citations
82
OpenCitations
 
94

Bibliography


Similar publications



Contact ORBi