Article (Scientific journals)
iTRAQ-based analysis of changes in the cassava root proteome reveals pathways associated with post-harvest physiological deterioration.
Owiti, Judith; Grossmann, Jonas; Gehrig, Peter et al.
2011In Plant Journal, 67 (1), p. 145-56
Peer Reviewed verified by ORBi
 

Files


Full Text
owitietal2011.pdf
Publisher postprint (1.24 MB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
Ascorbate Peroxidases/metabolism; Carboxylic Ester Hydrolases/metabolism; Cell Wall/metabolism; Crops, Agricultural; Databases, Protein; Down-Regulation/physiology; Gene Expression Regulation, Plant/physiology; Glucan 1,3-beta-Glucosidase/metabolism; Manihot/enzymology/metabolism/physiology; Plant Immunity/physiology; Plant Proteins/classification/metabolism; Plant Roots/metabolism; Proteome/metabolism; Proteomics/methods; Reactive Oxygen Species/metabolism; Signal Transduction/physiology; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods; Superoxide Dismutase/metabolism; Up-Regulation/physiology
Abstract :
[en] The short storage life of harvested cassava roots is an important constraint that limits the full potential of cassava as a commercial food crop in developing countries. We investigated the molecular changes during physiological deterioration of cassava root after harvesting using isobaric tags for relative and absolute quantification (iTRAQ) of proteins in soluble and non-soluble fractions prepared during a 96 h post-harvest time course. Combining bioinformatic approaches to reduce information redundancy for unsequenced or partially sequenced plant species, we established a comprehensive proteome map of the cassava root and identified quantitatively regulated proteins. Up-regulation of several key proteins confirmed that physiological deterioration of cassava root after harvesting is an active process, with 67 and 170 proteins, respectively, being up-regulated early and later after harvesting. This included regulated proteins that had not previously been associated with physiological deterioration after harvesting, such as linamarase, glutamic acid-rich protein, hydroxycinnamoyl transferase, glycine-rich RNA binding protein, beta-1,3-glucanase, pectin methylesterase, maturase K, dehydroascorbate reductase, allene oxide cyclase, and proteins involved in signal pathways. To confirm the regulation of these proteins, activity assays were performed for selected enzymes. Together, our results show that physiological deterioration after harvesting is a highly regulated complex process involving proteins that are potential candidates for biotechnology approaches to reduce such deterioration.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Owiti, Judith
Grossmann, Jonas
Gehrig, Peter
Dessimoz, Christophe
Laloi, Christophe
Hansen, Maria Benn
Gruissem, Wilhelm
Vanderschuren, Hervé  ;  Université de Liège - ULiège > Sciences agronomiques > Biologie végétale
Language :
English
Title :
iTRAQ-based analysis of changes in the cassava root proteome reveals pathways associated with post-harvest physiological deterioration.
Publication date :
2011
Journal title :
Plant Journal
ISSN :
0960-7412
eISSN :
1365-313X
Publisher :
Blackwell, Oxford, United Kingdom
Volume :
67
Issue :
1
Pages :
145-56
Peer reviewed :
Peer Reviewed verified by ORBi
Commentary :
(c) 2011 The Authors. The Plant Journal (c) 2011 Blackwell Publishing Ltd.
Available on ORBi :
since 06 October 2014

Statistics


Number of views
55 (0 by ULiège)
Number of downloads
0 (0 by ULiège)

Scopus citations®
 
98
Scopus citations®
without self-citations
92
OpenCitations
 
82

Bibliography


Similar publications



Contact ORBi