Article (Scientific journals)
Brief exposure of embryos to steroids or aromatase inhibitor induces sex reversal in Nile tilapia (Oreochromis niloticus)
Gennotte, Vincent; Mafwila Kinkela, Patrick; Ulysse, Bernard et al.
2015In Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology, 323, p. 31-38
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Keywords :
sex determination; sex differentiation; androgen; estrogen; sexual genotype
Abstract :
[en] This study aimed to develop sex reversal procedures targeting the embryonic period as tools to study the early steps of sex differentiation in Nile tilapia with XX, XY and YY sexual genotypes. XX eggs were exposed to masculinizing treatments with androgens (17α-methyltestosterone, 11-ketotestosterone) or aromatase inhibitor (Fadrozole), whereas XY and YY eggs were subjected to feminizing treatments with estrogen analog (17α-ethynylestradiol). All treatments consisted of a single or double 4-h immersion applied between 1 and 36 h post-fertilization (hpf). Concentrations of active substances were 1000 or 2000 µg l-1 in XX and XY, and 2000 or 6500 µg l-1 in YY. Masculinizing treatments of XX embryos achieved a maximal sex reversal rate of 10 % with an exposure at 24 hpf to 1000 µg l-1 of 11-ketotestosterone or to 2000 µg l-1 of Fadrozole. Feminization of XY embryos was more efficient and induced up to 91 % sex reversal with an exposure to 2000 µg l-1 of 17α-ethynylestradiol. Interestingly, similar treatments failed to reverse YY fish to females, suggesting either that a sex determinant linked to the Y chromosome prevents the female pathway when present in two copies, or that a gene present on the X chromosome is needed for the development of a female phenotype.
Disciplines :
Zoology
Author, co-author :
Gennotte, Vincent ;  Université de Liège - ULiège > Centre de formation et de recherche en aquaculture (CEFRA)
Mafwila Kinkela, Patrick 
Ulysse, Bernard
Akian Djétouan, Dieudonné
Bere Sompagnimdi, Frédéric
Tomson, Thomas
Mélard, Charles ;  Université de Liège - ULiège > Centre de formation et de recherche en aquaculture (CEFRA)
Rougeot, Carole ;  Université de Liège - ULiège > Centre de formation et de recherche en aquaculture (CEFRA)
Language :
English
Title :
Brief exposure of embryos to steroids or aromatase inhibitor induces sex reversal in Nile tilapia (Oreochromis niloticus)
Publication date :
2015
Journal title :
Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology
ISSN :
1932-5223
eISSN :
1932-5231
Publisher :
John Wiley & Sons, Hoboken, United States - New Jersey
Volume :
323
Pages :
31-38
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 03 October 2014

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