Short communication (Scientific journals)
Matrix ‐ assisted laser desorption/ionization mass spectrometry and Raman spectroscopy: An interesting complementary approach for lipid detection in biological tissues
Jadoul, Laure; Malherbe, Cédric; calligaris, David et al.
2014In European Journal of Lipid Science and Technology
Peer Reviewed verified by ORBi
 

Files


Full Text
Lipids-EJLST.pdf
Publisher postprint (749.68 kB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
Glycerophospholipids; MALDI MSI; Raman spectroscopy
Abstract :
[en] Recently, matrix‐assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) has emerged as a powerful technique to study the distribution of lipids. However, quantification still remains a challenge because the MALDI signal is strongly affected by ion suppression effects. On the contrary, Raman spectroscopy is recognized as a non‐destructive analysis method and spectral images can also be acquired. The combination of these two techniques was applied for lipids detection in tissue sections. In MALDI, two lipids families (glycerophosphocholine, PC; gycerophosphoethanolamine, PE), three MALDI matrices (1,5‐diaminonapthalene, 1,5‐DAN; 2,5‐dihydroxybenzoic acid, 2,5‐DHB; a‐4‐hydroxicinammic acid, CHCA), and various mixtures of lipids were investigated. The nature of the lipid, as well as the nature of the matrix and the composition of the sample influences the signal of a given lipid. In Raman, despite a strong overlap with the spectrum of the native tissue, an intensity profile constructed along the diameter of the section clearly shows that the signature of one given lipid (a glycerophosphocholine) can be detected on a doped biological sample.
Research center :
Mass Spectrometry Laboratory, Department of Chemistry, University of Liège, Liège, Belgium
Inorganic Analytical Chemistry Laboratory, Department of Chemistry, University of Liège, Liège, Belgium
Disciplines :
Anatomy (cytology, histology, embryology...) & physiology
Chemistry
Author, co-author :
Jadoul, Laure  ;  Université de Liège - ULiège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.)
Malherbe, Cédric  ;  Université de Liège - ULiège > Département de chimie (sciences) > Chimie analytique inorganique
calligaris, David
Longuespée, Rémi ;  Université de Liège - ULiège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.)
Gilbert, Bernard ;  Université de Liège - ULiège > Département de chimie (sciences) > Département de chimie (sciences)
Eppe, Gauthier  ;  Université de Liège - ULiège > Département de chimie (sciences) > Chimie analytique inorganique
De Pauw, Edwin  ;  Université de Liège - ULiège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.)
 These authors have contributed equally to this work.
Language :
English
Title :
Matrix ‐ assisted laser desorption/ionization mass spectrometry and Raman spectroscopy: An interesting complementary approach for lipid detection in biological tissues
Publication date :
2014
Journal title :
European Journal of Lipid Science and Technology
ISSN :
1438-7697
eISSN :
1438-9312
Publisher :
Wiley-VCH Verlag, Weinheim, Germany
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 10 September 2014

Statistics


Number of views
108 (21 by ULiège)
Number of downloads
8 (8 by ULiège)

Scopus citations®
 
5
Scopus citations®
without self-citations
3
OpenCitations
 
5

Bibliography


Similar publications



Contact ORBi