Identification and comparison of proteome patterns of English Grain Aphids on resistant and susceptible wheat

Some varieties of wheat are resistant to some aphid clones of the English Grain Aphid (Sitobion avenae). This study used 2D-DIGE coupled with protein identification by Maldi-Tof-MS to compare the proteome patterns of English Grain Aphids on resistant (Llareta) and susceptible (Huayún) wheat lines. Aphids were exposed to wheat during 2 weeks (short term: ST) and 4 weeks (long term: LT). Four treatments were compared: Llareta-ST, Llareta-LT, Huayún-ST, Huayún-LT. Experiment was conducted using different Sitobion avenae clones whose symbionts were previously characterized. Results showed that aphids had low performance on resistant plants. Seventy-two protein spots showed significant quantitative differences among the four treatment groups. Proteomic approach will allow a better understanding of interaction mechanisms between aphids and wheat.