Reference : Enantiomeric separation of basic compounds usin heptakis(2,3-di-O-methyl-6-O-sulfo)-b...
Scientific journals : Article
Human health sciences : Pharmacy, pharmacology & toxicology
http://hdl.handle.net/2268/17139
Enantiomeric separation of basic compounds usin heptakis(2,3-di-O-methyl-6-O-sulfo)-beta-cyclodextrin in combination with potassium camphorsulfonate in nonaqueous capillary electrophoresis: Optimization by means of an experimental design
English
Servais, Anne-Catherine mailto [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
Fillet, Marianne mailto [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
Chiap, Patrice mailto [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
Dewé, Walthère [Eli Lilly and Company > Lilly Development Centre > Statistical & Mathématical Sciences > >]
Hubert, Philippe mailto [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments > >]
Crommen, Jacques mailto [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
Aug-2004
Electrophoresis
Wiley-V C H Verlag Gmbh
25
16
2701-2710
Yes (verified by ORBi)
International
0173-0835
Weinheim
[en] basic drugs ; cyclodextrin ; ion-pairing reagent ; nonaqueous capillary electrophoresis
[en] The enantiomeric separation of a series of basic pharmaceuticals (beta-blockers, local anesthetics, sympathomimetics) has been investigated in nonaqueous capillary electrophoresis (NACE) systems using heptakis(2,3-di-O-methyl-6-O-sulfo)-beta-cyclodextrin (HDMS-P-CD) in combination with potassium camphorsulfonate (camphorSO(3)(-)). For this purpose, a face-centered central composite design with 11 experimental points was applied. The effect of the concentrations of HDMS-beta-CD and camphorSO(3)(-) on enantioresolution was statistically evaluated and depended largely on the considered analyte. The presence of camphorSO(3)(-) was found to be particularly useful for the enantioseparation of compounds with high affinity for the anionic CD. CamphorSO(3)(-) seems to act as a competitor, reducing the affinity for the CD, probably by ion-pair formation with these analytes. For compounds with lower affinity for HDMS-beta-CD, the combination of camphorSO(3)(-) and the CD appeared to have a favorable effect on enantioresolution only if the optimal CD concentration could be reached. On the other hand, for compounds characterized by a very low affinity for the anionic CD, the association of camphorSO(3)(-) and HDMS-beta-CD is always unfavorable. Finally, experimental conditions were selected by means of the multivariate approach in order to obtain the highest resolution (R-s) value for each studied compound.
Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS ; Fonds Léon Fredericq
http://hdl.handle.net/2268/17139
10.1002/elps.200405962

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