Article (Scientific journals)
Development of functionalized nanoparticles for vaccine delivery to dendritic cells: a mechanistic approach
Silva, Joana M.; Vandermeulen, Gaëlle; Oliveira, Vanessa G. et al.
2014In Nanomedicine, 9 (17), p. 2639-2656
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Keywords :
biomaterial; nanomedicine
Abstract :
[en] Aim: Produce biodegradable nanoparticles to target antigen-presenting cells and evaluate their potential to be used as a vaccine delivery system. Materials & methods: Untargeted PEGylated PLGA-based nanoparticles and mannose-grafted nanoparticles were formulated and physicochemically characterized. Immortalized and primary antigen-presenting cells were used to study nanoparticle internalization patterns. The endocytic pathways and intracellular trafficking followed by nanoparticles were also investigated. Results & discussion: Nanoparticles displayed mannose residues available for binding at the nanoparticle surface. Different nanoparticle internalization patterns by immortalized and primary antigen presenting cells were verified. Macropinocytosis, clathrin-mediated endocytosis, caveolin- and lipid raft-dependent endocytosis are involved in nanoparticles internalization. Nanoparticles demonstrate both endo-lysosomal and cytosolic localizations and a tendency to accumulate nearby the endoplasmic reticulum. Conclusion & future perspective: The developed nanoparticles might drive antigens to be presented through MHC class I and II molecules to both CD8+ and CD4+ T cells, favoring a complete and coordinated immune response.
Research center :
Center for Education and Research on Macromolecules (CERM)
Disciplines :
Chemistry
Materials science & engineering
Author, co-author :
Silva, Joana M.;  Universidade de Lisboa, Instituto de Investigação do Medicamento, Faculdade de Farmácia > Université catholique de Louvain (UCL), Brussels, Louvain Drug Research Institute (LDRI), Advanced Drug Delivery & Biomaterials
Vandermeulen, Gaëlle;  Université catholique de Louvain (UCL), Brussels > Louvain Drug Research Institute (LDRI) > Advanced Drug Delivery & Biomaterials
Oliveira, Vanessa G.;  Faculdade de Medicina da Universidade de Lisboa, Instituto de Medicina Molecular
Pinto, Sandra N.;  Universidade de Lisboa, Centro de Química-Física Molecular & Institute of Nanoscience and Nanotechnology, Instituto Superior Técnico
Rodrigues, Catarina;  Universidade de Lisboa, Instituto de Investigação do Medicamento, Faculdade de Farmácia
Salgado, Ana;  Universidade de Lisboa, Instituto de Investigação do Medicamento, Faculdade de Farmácia
Afonso, Carlos A.;  Universidade de Lisboa, Instituto de Investigação do Medicamento, Faculdade de Farmácia
Viana, Ana S.;  Faculdade de Ciências da Universidade de Lisboa, Centro de Química e Bioquímica
Jérôme, Christine  ;  Université de Liège - ULiège > Département de chimie (sciences) > Chimie des macromolécules et des matériaux organiques (CERM)
Silva, Liana C.;  Universidade de Lisboa, Instituto de Investigação do Medicamento, Faculdade de Farmácia
Graca, Luiis;  Faculdade de Medicina da Universidade de Lisboa, Instituto de Medicina Molecular
Préat, Véronique;  Université catholique de Louvain (UCL), Brussels > Louvain Drug Research Institute (LDRI) > Advanced Drug Delivery & Biomaterials
Florindo, Helena F.;  Universidade de Lisboa, Instituto de Investigação do Medicamento, Faculdade de Farmácia
More authors (3 more) Less
Language :
English
Title :
Development of functionalized nanoparticles for vaccine delivery to dendritic cells: a mechanistic approach
Publication date :
2014
Journal title :
Nanomedicine
ISSN :
1743-5889
eISSN :
1748-6963
Publisher :
Future Medicine Ltd
Volume :
9
Issue :
17
Pages :
2639-2656
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 18 July 2014

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