[en] Singlet oxygen (1O2), a reactive oxygen species, has been found to be implicated in many cellular events and pathological disorders.Herein, we investigated the reactivity of 1O2 towards the anaesthetic agent propofol (PPF) encapsulated within DMPC liposomes. By time
resolved luminescence, the rate constant of 1O2 quenching by PPF was evaluated, depending on the location of the sensitizer. The nature of the oxidation product, resulting from the reaction of
1O2 with PPF, was determined using absorption and HPLC techniques. Finally, the in vitro protective effect of PPF towards the1O2-induced neuronal cell toxicity was evaluated in terms of cell viability.
Disciplines :
Physics
Author, co-author :
Heyne, Belinda
Brault, Daniel
Fontaine-Aupart, Marie-Pierre
Kohnen, Stéphane
Tfidel, Francis
Mouithys-Mickalad, Ange ; Université de Liège - ULiège > Centre de l'oxygène : Recherche et développement (C.O.R.D.)
Dupont, Ginette
Hoebeke, Maryse ; Université de Liège - ULiège > Département de physique > Spectroscopie biomédicale
Language :
English
Title :
Reactivity towards singlet oxygen of propofol inside liposomes and neuronal cells
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